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Thank you for visiting our booth at 8th Annual DUNES Symposium

A big thank you to everyone who visited our booth at DUNES Symposium on 28th October 2016!

We hope you enjoyed the Halloween treats from us.

It was a great opportunity for us to showcase our products and new promotions.

We look forward to seeing you again!

Thank you everyone for visiting us at Biotech Fest, Biopolis Matrix

A big thank you to everyone who visited our booth at Biotech Fest held at Biopolis Matrix on 10th & 11th November 2016!

We are very happy that most of you had lots of fun!
Congratulations to those who won a plush toy home.

It was a great opportunity for us to showcase our products and new promotions.

We look forward to seeing you again!

Congratulations to our NGS Project Winners!

Axil Scientific would like to thank all researchers who have participated in our 3rd Birthday contest.
Due to overwhelming responses, we are very happy to pick not 1, but 3 winners to thank you for your support!

Congratulations to the 3 winners who will have their NGS project run for FREE!

Winner #1: Yang Yi
National University of Singapore
Dept of Civil & Environmental Engineering
PI: Prof. Martin Reinhard

Winner #2:Siti Sarah Daud
National University of Singapore
Dept of Paediatrics
PI: Dr Makoto Yawata

Winner #3: Aaron Irving
Duke-NUS
Emerging Infectious Diseases Programme
PI: Prof. Wang Linfa

Contact us at ngs@axilscientific.com for your NGS projects and enjoy a special price during this promotion period!

Multiplex Apoptosis and Necrosis

Multiplex Apoptosis and Necrosis

Apoptosis is an active, programmed process of autonomous cellular dismantling that avoids eliciting inflammation. In apoptosis, phosphatidylserine (PS) is transferred to the outer leaflet of the plasma membrane. As a universal indicator of the initial/intermediate stages of cell apoptosis, the appearance of phosphatidylserine on the cell surface can be detected before morphological changes are observed.

Necrosis is characterized as a passive, accidental cell death resulting from environmental perturbations with uncontrolled release of inflammatory cellular contents. Loss of plasma membrane integrity represents a straightforward approach to demonstrate late stage apoptosis and necrosis.

Cell Meter™ Apoptotic and Necrotic Detection Kits are a set of tools for monitoring cell viability. The kits are optimized to simultaneously detect cell apoptosis, necrosis and healthy cells with a flow cytometer or fluorescence microscope. The phosphatidylserine (PS) sensor used in Kit 22843 has deep red fluorescence (Ex/Em = 630/660 nm) upon binding to membrane PS. Membrane-impermeable Nuclear Green™ DCS1 (Ex/Em = 490/525 nm) is used to label the nucleus while CytoCalcein™ Violet 450 (Ex/Em = 405/450 nm) is provided for labeling live cell cytoplasm.

Key Features of Cell Meter™ Apoptotic and Necrotic Detection Kits (pg 15-16 of this catalog):

• Multiplexing capability, triple colors for the simultaneous detection of multiple cellular events.
• Robust, a mix and read format.
• Convenient, compatible with common filter sets.

The fluorescence images of Jurkat cells showing cells that are alive (blue, stained by CytoCalcein™ Violet 450, Cat# 22012), apoptotic (green, stained by Apopxin™ Green), and necrotic (red, indicated by 7-AAD staining, Cat# 17501). The fluorescence images of the cells were taken with Olympus fluorescence microscope in the Violet, FITC and TRITC channel respectively. Individual images taken in each channel from the same cell population were merged as shown above. B. Non-induced control cells. C: Triple staining of staurosporine-induced cells (1 μM staurosporine treatment for 3 hours.)

The detection of apoptosis and necrosis with Kit 22843. Binding activity of Apopxin™ Deep Red to phosphatidylserine in Jurkat cells. The fluorescence imaging demenstrated that live cells (blue) were stained by CytoCalcein™ Violet 450 (Cat# 22012), apoptotic cells (red) were stained by Apopxin™ Deep Red, and necrotic cells (green) were stained by Nuclear Green™ DCS1 (Cat# 17550). Apoptosis was induced by 1 μM staurosporine for 3 hours. The fluorescence images of the cells were taken with Olympus fluorescence microscope using the Violet, Cy5® and FITC channel respectively. Individual images taken in each channel from the same cell population were merged as shown above. A: Non-induced control cells; B: Triple staining of staurosporine-induced cells.

Product Ordering Information:

Cat# 22840 Cell Meter™ Apoptotic and Necrotic Detection Kit *Triple Fluorescence Colors*
Cat# 22843 Cell Meter™ Apoptotic and Necrotic Detection Kit *Triple Fluorescence Colors*

Nacalai Tesque: Bullet CBB Stain One Video: One-step staining in 15 minutes

Biochemical Newsletter
Fast CBB Staining in 15 min !

Bullet CBB Stain One (Ready to Use)

new

Bullet CBB Stain One is our simplest and fastest product for protein electrophoresis gel staining using Coomassie Brilliant Blue (CBB).
 

Product Image
  • Fast staining: One-step staining in 15 minutes
  • Easy to use: No need for pretreatment, washing, destaining or microwaving
  • Safety: No harmful solvents such as methanol or acetic acid
  • Highly sensitive: As little as several tens of ng of protein (BSA) can be detected

Video

Bullet CBB Stain One: One-step staining in 15 minutes.
Comparison with conventional coomassie blue staining

Video images

 

>> Learn More about Bullet CBB Stain One (Ready to Use)
>> Download PDF files (0.8MB)

 

Product name
 
Product
number
 
PKG size
 
Bullet CBB Stain One(Ready To Use)
 
 
50ML
Bullet CBB Stain One(Ready To Use)
 
 
500ML
Bullet CBB Stain One(Ready To Use)
 
 
1L

Sygnis - TruePrime™ liquid biopsy

Click here to find out more.

Citation Spotlight: p300 Lysine Acetyltransferase and Skeletal Muscle

Lysine Acetyltransferase p300 and Its Role in
Skeletal Muscle Biology
 

 

Recently, LaBarge et al. examined the role of the acetyltransferase E1a-binding protein (p300) in skeletal muscle function and metabolism. Reversible acetylation, a well-known post-translational modification, is considered a regulator of mitochondrial metabolism and exercise-induced adaptation in skeletal muscles. This conclusion is based primarily on data derived from studies of deacetylases and skeletal muscle physiology with a dearth of information on how lysine acetyltransferases impact the same muscle physiology. While whole-body heterozygous and homozygous p300 knockout mice have muscle defects (along with neural and cardiac) and die in embryogenesis, to date, the role of p300 in skeletal muscle function has not been studied with a muscle-specific knock-out mouse model. Here, the authors created such an in vivo model and found that knocking out p300 affected neither the development nor function of adult skeletal muscle. Moreover, it was also not required for mitochondrial adaptation induced by endurance exercising. Cytoskeleton's anti-acetyl lysine antibody (Cat. # AAC01) was an essential reagent in this study as it was used to confirm a functional loss of p300 in the knock-out mice. These mice had a significant reduction in total acetylation levels in skeletal muscle immunoprecipitates from knock-out, compared to wild-type, mice. 

Find out more.

Check out Sygnis at the upcoming renowed scientific conferences!

Sygnis will be presenting its revolutionary novel multi displacement amplification (MDA) technology TruePrime™ at the upcoming renowned scientific conferences:

  • Molecular Medicine Tri Conference (Molecular Med TRI-CON), on March 6-11, San Francisco, CA, USA.
  • 10th International Symposium on Minimal Residual Cancer: Liquid Biopsy in Cancer Diagnostics and Treatment (ISMRC), on March 19-21, Hamburg, Germany.

​Find out more.

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