New products

Bullet PAGE One Precast Gel

Manufacturer: 
Specifications: 

Features

  • Only 10 minutes with 400 V
  • High transfer efficiency of proteins on western blot membrane
  • Works well in conventional Laemmli running buffer and sample buffer
  • 17-well gel is usable with multichannel pipet for consistent loading

>> Click here for the video of Bullet PAGE One Precast Gel vs Laemmli gel 16x

Performance Comparison
Bullet PAGE One Precast Gel can produce an excellent separation image with the shortest electrophoresis.

Product Name   Bullet PAGE One Company A  Company B 
Gel %  5 - 15%  4 - 15% 4 - 12% 
Product No.   13079-84  -
Electrophoresis Time 11 min.   33 min. 50 min. 
Electrophoresis
Images 
     

<Electrophoresis condition>

Sample:   Protein Markers(10x)(Prod No. 29458-24), 5 µl
Gel Staining:   CBB Stain One (Prod No. 04543)
Voltage Constant:  Bullet PAGE One: 400V, Company A and Company B: 200V

Comparison of Protein Transfer Efficiency

Bullet PAGE One Precast Gel indicates obviously higher protein transfer efficiency than company A’s, even though blotting time was only 10 min.

<Sample>
① Protein Ladder One(Triple-color)(Product No. : 09547-74)
② Protein Markers(3x) 2 µl added
④ Protein Markers(1x) 2 µl added
⑤ Protein Markers(1/3) 2 µl added
<Transfer>
Use Semi ‐ dry Blotting Buffer Solution for Western Blotting (Product No.: 30650-31)
(Transfer time : 10 min. and 20 min. 10V)
<PVDF membrane staining>
Stain PVDF membrane with CBB Stain One (Product No. : 04543).

 

Gel Types

Gel Gradient Gel Single Percentage Gel
 Gel % 5 - 11% 5 - 15% 6% 8%
Product No. 13 well
17 well 
13077-04
13078-94
13079-84
13080-44
13081-34
13082-24
13083-14
13084-04
Electrophoresis Images

Product Specification

Glass Plate Size:  W100mm x H80mm x T3.2mm 
Gel Size:   W80mm x H60mm x T1.0mm 
Sample Well Configuration / Maximum Load Volume:  13-well / 40 µl, 17-well / 28 µl

Cell Emigre

Manufacturer: 
Specifications: 

A quick & quantitative assay for determining linear cell migration rate.
It is designed for use with attachment-dependent mammalian cells.

What can Cell Émigré be used for?

  • Cell Émigré is designed to measure the linear migration rate of attachment-dependent mammalian cells.
  • Migration rate responses can be readily compared across different cell types. The assay provides a rapid means of evaluating and comparing the effect of chemical or biological treatments such as chemical agents, proteins, peptides, antibodies or genetically modified cells.

What are the advantages of Cell Émigré?

1. AN EASY-TO-USE ASSAY

The end-user supplies a cell sample (of freshly detached cells in fluid suspension) to a microfluidic ‘chip’. The chip then automatically distributes cells to microchannel arrays – ready for attachment & migration. No pumps or external fluid connections are required; each chip contains a reservoir for culture medium which is filled during the initial cell addition step.

2. MEASURES THE RATE OF LINEAR CELL MIGRATION – NO MORE GOING IN CIRCLES!

Existing 2D-migration assays often attempt to measure ‘random-walk’ cell migration – a challenging task! Cell Émigré takes the innovative approach of quantifying migration within an array of linear microchannels. Each cell under assay is constrained to a linear migration path through contact-guided migration along a microchannel wall.

3. RAPID MIGRATION QUANTIFICATION

Cells begin migrating within Cell Émigré aligned to a common START position; a cells displacement from this position indicates its linear migration distance. There is no need for video recording or computer image analysis.

Each microchannel in the array contains a parallel measurement scale calibrated in microns. Cell migration can be rapidly & easily quantified (to the nearest 50μm) by visually comparing the alignment of a cell within the microchannel with this integrated measurement scale (using x40-x100 microscopic examination). Migration can be quantified at any time point during an assay.

Results may be expressed as a migration rate: microns per hour (μm/h).

4. REDUCED REAGENT CONSUMPTION

Achieved through the use of microfluidic technology. A single chip containing 3x assays consumes <350μl of fluid when loaded. This reduces costs in terms of cells and reagents.

Product and Supporting Material

For more information, please visit:
http://www.biocolor.co.uk/index.php/assay-kits/cell-emigre-cell-migration-assay

DNA Sequencing

Manufacturer: 
Specifications: 

We use Applied Biosystems' highest capacity-based genetic analyzer platforms and optimized protocols to give you superior data in the shortest turnaround time. Currently we are using the BigDye® Terminator v3.1 cycle sequencing kit chemistry, as its robust nature makes it highly suitable for a variety of applications, from de novo sequencing, resequencing and finishing PCR products.

Be assured that your DNA template is in good hands. We work hard to make sure we continuously meet or exceed leading industry providers in terms of QV, trace score and contiguous read length. Our experienced team ensures you get quality results, even with difficult templates, and prompt troubleshooting assistance at all times.

Features of 1st BASE DNA Sequencing Services:

  • High quality, long read lengths with fast turnaround time
  • Wide range of free sequencing primers for ABI's dye terminator chemistry
  • 1st BASE DNA Sequencing Collection Kit available for ease of organizing DNA templates/primers
  • Email notification of sequencing results, electropherogram (in .ab1 format) and sequences (in .seq fasta format)
  • FREE re-run of failed reaction(s)

High Protein Yield - NEW: FectoPRO™

Manufacturer: 
Specifications: 



 

FectoPROTM gives amazing antibody production yields in suspension CHO cells.
Cells were seeded at 1 x 106 cells/ml in 30ml of FreestyleTM CHO Expression Medium and transfected with FectoPROTM (0.8μg DNA/ml) or FreeStyleTM MAX Reagent (1.25μg DNA/,ml)
following recommended protocols for the respective reagents. Recombinant mouse lgG production yield was assayed 1 to 6 days after transfection using protein G biosensor (fortéBIO® octet RED96 system).

Data kindly provided by ProteoGenix SAS (France)

FectoPROTM gives high transfection efficiency in suspension CHO cells.
FreeStyleTM CHO-S cells were seeded at 1 x 106 cells/ml in 30 ml of FreeStyleTM CHO Expression Medium and transfected with FectoPROTM + FectoPROTM Booster of FreeStyleTM MAX Reagent using 0.4μg/ml and 1.25μg/,l of a GFP expressing plasmid, respectively. GFP expression was assayed 24 h after transfection using fluorescence microscopy.

FectoPROTM gives superior protein prodcuction yields in suspension CHO and HEK-293 cells.
FreeStyleTM CHO-S and HEK-293F cells were seeded at 1 x 106 cells/ml in 30 ml of FreeStyleTM CHO or 293 Expression Medium and transfected with FectioPROTM + FectoPROTM Booster (0.4μg DNA/ml), FreeStyle TM MAx Reagent (1.25μg DNA/ml) or PEIproTM (1μg DNA/ml) following recommended protocols for the respective reagents. IgG3-Fc (34kDa) production was assayed 72 h (CHO cells) and 120 h (HEK-293 cells) after transfection by protein G affinity quantification (HPLC) and Western Blot. Standard corresponds to purified IgG3-Fc (34kDa).

FectoPROTM HEK-293F cells were seeded at 1 x 106 cells/ml in 30 ml of FreeStyleTM 293 Expression Medium and transfected with FectoPROTM + FectoPROTM Booster (0.5 μg DNA/,l) following the standards protocol. IgG3-Fc production was assayed at different day by protein G affinity quantification (HPLC). Similar results are obtained with FreeStyleTM CHO-S (data not shown).

FectoPROTM is easily scalable and reproducible in CHO cells.
FreeStyleTM CHO-S cells were seeded at 1 x 106 cells/ml in 30ml, 100ml, 300ml or 1L of FreeStyleTM CHO Expression Medium and transfected with FectoPROTM + FectoPROTM Booster (0.5μg DNA/ml). At day 1 to 6, cell viability was assayed using Trypan blue exclusion assay (A) and IgG3-Fc production was assayed by protein G affinity quantification (HPLC) (B) and Western Blot (C). Standard (C) corresponds to purified IgG3-Fc (34 kDa).

Suspension CHO cells were seeded at 1 x 106 cells/ml in 30 ml of CHO medium and transfected using 3 different lots of FectoPROTM (CCIII 078, CCIII 104 or CCIII 106) following the standard protocol. 72 h after transfection, IgG3-Fc production was assayed by protein G affinity quantification. Similar results are obtained on suspension HEK-2933 cells (data not shown).

FectoPROTM gives superior protein production yields in suspension CHO cells grown in different media. CHO cells were seeded at 1 x 106 cells/ml in 30 ml of FreeStyleTM CHO Expression Medium, CD CHO Medium or Pro-CHOTM 4 Medium and transfected with FectoPROTM + FectPROTM Booster or FreeStyleTM MAX Reagent following recommended protocols for the respective reagents. IgG3-Fc production was assayed 72 h after transfection by protein G affinity quantification (HPLC).

jetCRISPR™ - RNP transfection reagent for genome editing

Manufacturer: 
Specifications: 

 

For more information on jetCRISPR™, click here.

Molecular Biology Reagents

Manufacturer: 
Specifications: 

1st BASE launches their range of Molecular Biology Reagents with new products and more product enhancements.

High Performance PCR Reagents

Product Information

Product No.

Description

BIO-5110-500U

Taq DNA Polymerase (recombinant), 500U

BIO-5120-1ml

dNTP Mix, 10mM each, 1.0ml

BIO-5125-1ml

dNTP Set, 100mM, 4 X 0.25ml

Products for DNA Electrophoresis 

Product Information

Product No.

Description

BIO-5130-50ug

100bp DNA Ladder, Ready-To-Use, 50ug

BIO-5130-100ug

100bp DNA Ladder, Ready-To-Use, 100ug

BIO-5140-50ug

1kb DNA Ladder, Ready-To-Use, 50ug

BIO-5140-100ug

1kb DNA Ladder, Ready-To-Use, 100ug

BIO-5160-6ml

6X DNA Loading Dye, 3-Colour, 6 X 1ml

BIO-5170-1ml

DNA Stain, 1ml

 

Prestained Protein Ladder

Broad range protein ladder with an Orange reference band at ~75kDa and a Green reference band at ~25kDa

Product Information

Product No.

Description

BIO-5150-250ul

Prestained Protein Ladder, 3-Colour, 250ul

BIO-5150-500ul

Prestained Protein Ladder, 3-Colour, 500ul

New Products

Manufacturer: 
Specifications: 

Click here to view AllCells' New Products.

Newly Added Growth Factors

Manufacturer: 
Specifications: 

Growth factors and stem cell reagents have an important role in cancer and developmental research. GoldBio’s growth factors have continued to explode on the marketplace, which is why they are so excited to announce that they are expanding this product line! Currently, more than 100 new growth factors have been added to their pre-existing list, and they plan to add more throughout the year.So stay tuned for more great offers!

They have added new EGFs, PDGFs, Cytokines, FGFs, Interleukins, and Neurotrophic factors as well as a completely new category: Interferons. These additions were based on your feedback and requests and they’re excited that they can provide you with even more of the products they need at the same great rates that GoldBio has been providing for years!

ALSO SEE THESE EXPANDED OR NEW CATEGORIES:

FGFS

The FGF family of proteins is one of the largest polypeptide growth factor families in the animal kingdom, found in organisms ranging from nematodes to humans. FGFs are involved with embryonic development, angiogenesis, wound healing, metabolic regulation and cancer. GoldBio’s selection of growth factors includes 25 FGFs, with the line continuing to grow. Among the newly added FGFs are FGF4, FGF13, FGF19 and FGF21.

INTERLEUKINS

Interleukins are a large family of cytokines which are soluble hormone-like mediators of the immune system. They involved in processes of cell activation, cell differentiation, proliferation and cell-to-cell interactions. The expression of interleukins is strictly regulated. For example, the factors are often not secreted constitutively. GoldBio offers a large catalog of interleukins, with new additions: IL1A, IL1RN, IL2 Cys141Ser, IL5, IL8, IL9,IL17B, IL21 and IL36B.

NEUROTROPHIC FACTORS

Neurotrophic factors are involved in many roles of the CNS beyond growth and development. They are responsible for the maintenance and survival of neurons and have been the subject of extensive research. There is continued interest in the research of potential neurotrophic factor therapies, which may help treat Alzheimer’s disease, Parkinson’s disease and other neurodegenerative disorders. GoldBio offers high-quality recombinant neurotrophic factors. Our newest additions to the line include CDNF, CNTF, GMFB, MANF, MDK, NRG1-alpha, NRG1-beta1, NRG1-beta2, NRN1, PSPN and PTN.

INTERFERONS (NEW CATEGORY)

Interferons are a family of cytokine proteins that play an important role in modulating immune response. They are secreted in response to viruses and other pathogens, triggering such responses that prevent viral replication, activation immune cells, inhibit cell division and affect cell differentiation. There are three classes of interferons: Type I, Type II and Type III IFN which relate to the type of receptor through which the IFN signal. GoldBio is excited to offer a variety of each IFN of each type, including: IFN-alpha1A, IFN-alpha1B, IFN-alpha2A, IFN-alpha2B, IFN-beta1B, IFN-gamma, IFN-lambda1, IFN-lambda2, IFN-lambda3 and IFN-omega1.

Smooth Muscle Cells (7 Types)

Specifications: 

Lifeline Now has 7 Different Types of Normal Human Smooth Muscle Cells! 

Lifeline’s Smooth Muscle Cells and VascuLife SMC Media have been cited in 12 recent publications.
See our Reference Page for more information

Get better results with Lifeline's Superior Normal Human Smooth Muscle Cells

Lifeline’s Smooth Muscle Cells are isolated from the Aorta, the Pulmonary Artery, the Coronary Artery, from Bronchial Tracheal tissue, from the Lungs, from the Bladder, and from the Uterus. Each smooth muscle cell product demonstrates excellent morphology and growth in our fully optimized VascuLife SMC Media

When Lifeline's Human Smooth Muscle Cells are grown in VascuLife SMC optimized medium, our cell growth far exceeds the competition. Lifeline's Human Smooth Muscle Cell Systems provide an ideal low serum (5%) culture system, ideal for the study of: Angiogenesis; Atherosclerosis; Diabetes; Pulmonary Arterial Hypertension; Asthma; and Bladder Disorders and Uterine Contractibility Studies 

Lifeline's Smooth Muscle Cell Systems
Human Uterine Smooth Muscle Cell Systems, (HUtSMC) 
Human Aortic Smooth Muscle Cells, (HAoSMC) 
Human Pulmonary Artery Smooth Muscle Cells, (HPASMC) 
Human Coronary Artery Smooth Muscle Cells, (HCASMC) 
Human Lung Smooth Muscle Cells, (HLSMC) 
Bronchial/Tracheal Smooth Muscle Cells, (HBTSMC)
Human Bladder Smooth Muscle Cells, (HBSMC) 
VascuLife SMC Complete Kit 
FrostaLife Cryopreservation Solution ; 100mL 
TrypKit , 100mL 

VeriPlex Multiplex ELISAs

Manufacturer: 
Specifications: 

PBL Assay Science’s VeriPlex ELISAs are fully quantitative, high throughput multiplex ELISA kits that provide simultaneous quantification and detection of up to 16 different analytes from a single sample. PBL’s multiplex kits allow evaluation of the levels of one given inflammatory molecule in the context of several others, repeated measurements of the same cytokine panels in the same subjects under the same experimental assay conditions, and reliable detection of different proteins across a broad dynamic range of concentrations.

This kit provides unique chemiluminescent results on each analyte within a sample and allows for a global understanding of ongoing immune responses in diseases ranging from cancer to autoimmunity.

Features/Benefits:

  • Customizable array of analytes including Type I, II & III IFNs, Interleukins and more
  • Simultaneous quantification of up to 16 pro- & anti-inflammatory cytokines in a single 50 µl sample
  • Provides analyte sensitivity as low as 5 pg/ml
  • Cost effective multiplex platform requires only a common CCD imager system
  • Reproducible results in autoimmune serum, plasma and tissue culture media (TCM)

Multiplex vs. Traditional ELISA

Category    Multiplex Technology  Traditional ELISA
Sample Size 15-50 µl / well 50-100 µl / well
Sample Type  Many types  Many types 
Protocol Modified ELISA  Standardized
Time   3 hours for 2400 data points 48 hours for 2400 data points
Data Output 2400 data points / test 96 data points / test
Cost Lower Cost- $1.30 / data point  High Cost- $5 / Data point
Sensitivity Higher Sensitivity Lower Sensitivity
Availability Increasing Product Offerings Wide Range of Products
Equipment Q-View Imager less than $15k  Various

Specific Product Information

51500-1 VeriPlex Human Interferon 9-Plex ELISA Kit

51510-1 VeriPlex Human Cytokine 16-Plex ELISA Kit

Download flyers here for 16-Plex and 9-Plex.

For more information, please click here.