Products spotlight

Animal Pathogens

Specifications: 

 

Reagents available for:
Mice - Rat - Guinea Pig - Hamster - Rabbit - Canine - Feline - Simian

Bullet PAGE One Precast Gel

Manufacturer: 
Specifications: 

Features

  • Only 10 minutes with 400 V
  • High transfer efficiency of proteins on western blot membrane
  • Works well in conventional Laemmli running buffer and sample buffer
  • 17-well gel is usable with multichannel pipet for consistent loading

>> Click here for the video of Bullet PAGE One Precast Gel vs Laemmli gel 16x

Performance Comparison
Bullet PAGE One Precast Gel can produce an excellent separation image with the shortest electrophoresis.

Product Name   Bullet PAGE One Company A  Company B 
Gel %  5 - 15%  4 - 15% 4 - 12% 
Product No.   13079-84  -
Electrophoresis Time 11 min.   33 min. 50 min. 
Electrophoresis
Images 
     

<Electrophoresis condition>

Sample:   Protein Markers(10x)(Prod No. 29458-24), 5 µl
Gel Staining:   CBB Stain One (Prod No. 04543)
Voltage Constant:  Bullet PAGE One: 400V, Company A and Company B: 200V

Comparison of Protein Transfer Efficiency

Bullet PAGE One Precast Gel indicates obviously higher protein transfer efficiency than company A’s, even though blotting time was only 10 min.

<Sample>
① Protein Ladder One(Triple-color)(Product No. : 09547-74)
② Protein Markers(3x) 2 µl added
④ Protein Markers(1x) 2 µl added
⑤ Protein Markers(1/3) 2 µl added
<Transfer>
Use Semi ‐ dry Blotting Buffer Solution for Western Blotting (Product No.: 30650-31)
(Transfer time : 10 min. and 20 min. 10V)
<PVDF membrane staining>
Stain PVDF membrane with CBB Stain One (Product No. : 04543).

 

Gel Types

Gel Gradient Gel Single Percentage Gel
 Gel % 5 - 11% 5 - 15% 6% 8%
Product No. 13 well
17 well 
13077-04
13078-94
13079-84
13080-44
13081-34
13082-24
13083-14
13084-04
Electrophoresis Images

Product Specification

Glass Plate Size:  W100mm x H80mm x T3.2mm 
Gel Size:   W80mm x H60mm x T1.0mm 
Sample Well Configuration / Maximum Load Volume:  13-well / 40 µl, 17-well / 28 µl

Cell Emigre

Manufacturer: 
Specifications: 

A quick & quantitative assay for determining linear cell migration rate.
It is designed for use with attachment-dependent mammalian cells.

What can Cell Émigré be used for?

  • Cell Émigré is designed to measure the linear migration rate of attachment-dependent mammalian cells.
  • Migration rate responses can be readily compared across different cell types. The assay provides a rapid means of evaluating and comparing the effect of chemical or biological treatments such as chemical agents, proteins, peptides, antibodies or genetically modified cells.

What are the advantages of Cell Émigré?

1. AN EASY-TO-USE ASSAY

The end-user supplies a cell sample (of freshly detached cells in fluid suspension) to a microfluidic ‘chip’. The chip then automatically distributes cells to microchannel arrays – ready for attachment & migration. No pumps or external fluid connections are required; each chip contains a reservoir for culture medium which is filled during the initial cell addition step.

2. MEASURES THE RATE OF LINEAR CELL MIGRATION – NO MORE GOING IN CIRCLES!

Existing 2D-migration assays often attempt to measure ‘random-walk’ cell migration – a challenging task! Cell Émigré takes the innovative approach of quantifying migration within an array of linear microchannels. Each cell under assay is constrained to a linear migration path through contact-guided migration along a microchannel wall.

3. RAPID MIGRATION QUANTIFICATION

Cells begin migrating within Cell Émigré aligned to a common START position; a cells displacement from this position indicates its linear migration distance. There is no need for video recording or computer image analysis.

Each microchannel in the array contains a parallel measurement scale calibrated in microns. Cell migration can be rapidly & easily quantified (to the nearest 50μm) by visually comparing the alignment of a cell within the microchannel with this integrated measurement scale (using x40-x100 microscopic examination). Migration can be quantified at any time point during an assay.

Results may be expressed as a migration rate: microns per hour (μm/h).

4. REDUCED REAGENT CONSUMPTION

Achieved through the use of microfluidic technology. A single chip containing 3x assays consumes <350μl of fluid when loaded. This reduces costs in terms of cells and reagents.

Product and Supporting Material

For more information, please visit:
http://www.biocolor.co.uk/index.php/assay-kits/cell-emigre-cell-migration-assay

Coated Slides (Epoxysilane, Aldehydesilane, Aminosilane, or Hydrogel Coating)

Manufacturer: 
Specifications: 

Nexterion® Slide E is especially suitable for covalent immobilization of amino-modified and unmodified Oligonucleotides. The epoxy surface coating allows efficient and stable attachment of the biomolecules and provides optimal probe presentation. The nucleic acids react instantly with the epoxy modified surface to form a stable covalent bond. The density of epoxy-groups of the surface remains constant over the entire surface of the slide and has been adjusted to provide optimal binding capacity. The hydrophobic surface creates smaller spot diameters ideally suited for high-density arrays. The surface chemistry is stable and remains active even during very long spotting runs.

Recommended Probe Types:

  • Amino-modified and unmodified oligonucleotides
  • cDNA molecules and PCR products

Features/Benefits:

  • Stable and covalent binding of probes
  • Superior signal-to-background ratios even from weakly expressed genes
  • Hydrophobic coating supports the spotting of high-density arrays
  • Epoxy chemistry enables long spotting runs

A full range of functional coating chemistries for DNA and protein microarraying is available. Please contact our Product Specialist for your specific microarray application needs.

DNA for HTS application - jetPEI™

Manufacturer: 
Specifications: 

jetPEI™ transfection reagent is particularly well suited for automated or manual HTS (High Throughput Screening) as transfection with jetPEI™ is exceptionally efficient and reproducible. A distinctive linear polyethylenimine (PEI) derivative, jetPEI™ gently facilitates intracellular DNA delivery for high performance. 

Three HTS protocols are available depending on the experiment: forward, batch or reverse.

Figure: HEK-293 cells expressing GFP after transfection using jetPEI™.

Features/Benefits:

  • Fast and efficient methods to transfect cells for HTS
  • Compatible with serum and antibiotics
  • Robust jetPEI/DNA complexes
  • Exceptionally reproducible results
  • Well-suited for automated approaches

Specifications:

CATALOG # Amount of reagent Amount of NaCl solution
101-01N 0.1 mL 5mL
101-10N 1 mL 50 mL
101-10 1 mL -
101-40N 4 x 1mL 4 x 50 mL
101-40 4 x 1 mL -
101B-010N 10 mL 2 x 250 mL
101B-010 10 mL -

One ml of jetPEI™ is sufficient to perform 2 000 transfections in 96-well plates.

For more information on DNA for HTS application - jetPEI™, click here.

 

DNA in vitro - jetPRIME™

Manufacturer: 
Specifications: 

If you routinely perform transient gene expression studies and/or gene silencing by RNA interference, the new powerful jetPRIME™ is the reagent for you!

Transfection with this versatile reagent requires low quantities of nucleic acid and small amounts of reagent. Therefore, jetPRIME™ is both gentle to cells and economical. A simple and convenient protocol ensures that it’s easy to scale up or scale down.

Features/Benefits:

  • Outstanding DNA transfection efficiency
  • Economical: less DNA and less reagent needed
  • Better cell viability
  • Excellent gene silencing
  • Cotransfection of DNA & siRNA
  • shRNA and miRNA plasmid transfection
  • Convenient protocol

Specifications:

Catalog Number Description
114-01

jetPRIME kit 0.1 ml of jetPRIME + 5 ml of jetPRIME buffer

114-07

jetPRIME kit 0.75 ml of jetPRIME + 40 ml of jetPRIME buffer

114-15

jetPRIME kit 1.5 ml of jetPRIME + 2 x (40 ml of jetPRIME buffer)

114-75

jetPRIME kit 5 x (1.5 ml of jetPRIME) + 1 x 55 ml of jetPRIME buffer 10x (conc.)

114-75C

jetPRIME kit 5 x (1.5 ml of jetPRIME) + 1 x 120 ml of jetPRIME buffer 5x (conc.)

1.5 ml is sufficient to perform ca.375 transfections in 6-well plates. Bulk quantities are available upon request

Contact us for a trial size of jetPRIME™ DNA & siRNA transfection reagent. Special offer with your first order of jetPRIME™!

For more information on jetPRIME™, click here.

Fetal Bovine sera

Manufacturer: 
Specifications: 

US Origin Fetal Bovine Serum (FBS) is the most widely used FBS in the world. Although US Origin FBS is considered the world’s standard for quality it is never the less becoming a lower standard in the view of the OIE (World Organization for Animal Health) and other regulatory bodies. On a scale of 1-4 for geographical BSE risk (GBR), with 1 being the lowest risk and 4 being the highest risk, US FBS ranks a 3 – demonstrating a moderate to high risk that BSE exists in the USA. US Origin FBS provided by J R Scientific is of the highest quality available in the USA. It is collected using state of the art aseptic collection techniques resulting in low endotoxin and hemoglobin concentrations. 

Finished Product Specifications

Test

Specifications

Endotoxin (Limulus Amebocyte Lysate – Gel Clot)

≤ 10 EU / ml

Hemoglobin (spectrophotometric)

≤ 20 mg / dl

Sterility Testing (current USP) for Bacteria and Fungi

Negative for growth

Virus Testing- Modified 9CFR

 

Flourescent Antibody

 

Bluetonque

Not Detected

Bovine Adenovirus

Not Detected

Bovine Parvovirus

Not Detected

Bovine Respiratory Syncytial Virus

Not Detected

Bovine Viral Diarrhea Virus

Not Detected

Rabies

Not Detected

Reovirus

Not Detected

Cytopathogenic Agents (IBR)

Not Detected

Hemadsorbing Agents (PI3)

Not Detected

Mycoplasma

 

Large Volume, Direct Culture

Not Detected

Description

Catalog number

Fetal Bovine Serum U.S Origin Premium (500ml)

43640-500

Fetal Bovine Serum U.S.D.A Premium (500ml)

43635-500

Fetal Bovine Serum N.Z Premium (500ml)

  43613-500 

Fetal Bovine Serum AU Premium (500ml)

43652-500 

 

For a full serum product listing, click here!

Growth Factors & Stem Cell Reagents

Manufacturer: 
Specifications: 

Gold Biotechnology is excited to offer an ever-increasing number of recombinant proteins for research. These growth factors include cytokines, fibroblast growth factors (FGFs), interleukins and neurotrophic factors and are originated from human, murine, rat and other assorted species. Our high-quality recombinant proteins have a purity level exceeding 95% in most cases and also have excellent bioactivity with low endotoxin levels. GoldBio recombinant proteins are typically derived from an E. coli source and are fully biologically active in mammalian cell culture. GoldBio’s growth factors provide your research with comparative activity but at just a fraction of the price of other commercially available recombinant proteins so that your research funds can be better focused on what really matters.

Recombinant Proteins - Complete List
Human Recombinant Proteins
Murine Recombinant Proteins
Rat Recombinant Proteins
Other Species Recombinant Proteins
Amyloids
BMP & Noggin
Chemokines
Cytokines
EGF
FGF
Interferons
Interleukins
Lymphoid/Lymphocyte
Neurotrophic Factors
PDGF & VEGF
Peptide Hormones
SHH

 

Contact your Product Specialist or email us at custcare@axilscientific.com to enquire.

For more information, please visit www.goldbio.com/category/growth-factors-stem-cell-reagents.

High Protein Yield - NEW: FectoPRO™

Manufacturer: 
Specifications: 



 

FectoPROTM gives amazing antibody production yields in suspension CHO cells.
Cells were seeded at 1 x 106 cells/ml in 30ml of FreestyleTM CHO Expression Medium and transfected with FectoPROTM (0.8μg DNA/ml) or FreeStyleTM MAX Reagent (1.25μg DNA/,ml)
following recommended protocols for the respective reagents. Recombinant mouse lgG production yield was assayed 1 to 6 days after transfection using protein G biosensor (fortéBIO® octet RED96 system).

Data kindly provided by ProteoGenix SAS (France)

FectoPROTM gives high transfection efficiency in suspension CHO cells.
FreeStyleTM CHO-S cells were seeded at 1 x 106 cells/ml in 30 ml of FreeStyleTM CHO Expression Medium and transfected with FectoPROTM + FectoPROTM Booster of FreeStyleTM MAX Reagent using 0.4μg/ml and 1.25μg/,l of a GFP expressing plasmid, respectively. GFP expression was assayed 24 h after transfection using fluorescence microscopy.

FectoPROTM gives superior protein prodcuction yields in suspension CHO and HEK-293 cells.
FreeStyleTM CHO-S and HEK-293F cells were seeded at 1 x 106 cells/ml in 30 ml of FreeStyleTM CHO or 293 Expression Medium and transfected with FectioPROTM + FectoPROTM Booster (0.4μg DNA/ml), FreeStyle TM MAx Reagent (1.25μg DNA/ml) or PEIproTM (1μg DNA/ml) following recommended protocols for the respective reagents. IgG3-Fc (34kDa) production was assayed 72 h (CHO cells) and 120 h (HEK-293 cells) after transfection by protein G affinity quantification (HPLC) and Western Blot. Standard corresponds to purified IgG3-Fc (34kDa).

FectoPROTM HEK-293F cells were seeded at 1 x 106 cells/ml in 30 ml of FreeStyleTM 293 Expression Medium and transfected with FectoPROTM + FectoPROTM Booster (0.5 μg DNA/,l) following the standards protocol. IgG3-Fc production was assayed at different day by protein G affinity quantification (HPLC). Similar results are obtained with FreeStyleTM CHO-S (data not shown).

FectoPROTM is easily scalable and reproducible in CHO cells.
FreeStyleTM CHO-S cells were seeded at 1 x 106 cells/ml in 30ml, 100ml, 300ml or 1L of FreeStyleTM CHO Expression Medium and transfected with FectoPROTM + FectoPROTM Booster (0.5μg DNA/ml). At day 1 to 6, cell viability was assayed using Trypan blue exclusion assay (A) and IgG3-Fc production was assayed by protein G affinity quantification (HPLC) (B) and Western Blot (C). Standard (C) corresponds to purified IgG3-Fc (34 kDa).

Suspension CHO cells were seeded at 1 x 106 cells/ml in 30 ml of CHO medium and transfected using 3 different lots of FectoPROTM (CCIII 078, CCIII 104 or CCIII 106) following the standard protocol. 72 h after transfection, IgG3-Fc production was assayed by protein G affinity quantification. Similar results are obtained on suspension HEK-2933 cells (data not shown).

FectoPROTM gives superior protein production yields in suspension CHO cells grown in different media. CHO cells were seeded at 1 x 106 cells/ml in 30 ml of FreeStyleTM CHO Expression Medium, CD CHO Medium or Pro-CHOTM 4 Medium and transfected with FectoPROTM + FectPROTM Booster or FreeStyleTM MAX Reagent following recommended protocols for the respective reagents. IgG3-Fc production was assayed 72 h after transfection by protein G affinity quantification (HPLC).

Human Gingival Cells

Specifications: 

Introducing NEW Gingival Cells From Lifeline

Lifeline's Human Oral Keratinocytes (from Gingiva) and Human Gingival Fibroblast are cryopreserved as secondary cells to ensure the highest viability and plating efficiency.

The Human Oral Keratinocytes ( from Gingiva) are quality tested in DermaLife K Medium to ensure proper growth and morphology over a period of at least 15 population doublings.

The Human Gingival Fibroblast cells are quality tested in FibroLife Medium to ensure proper growth and morphology over a period of at least 15 population doublings.

Each of these cell systems provide models for studying Gingival wound healing, basic oral biology and toxicology. 

Related Gingival Products

FC-0094 , HOKg,  Normal Human Oral Keratinocytes from Gingiva
FC-0095 , HGF, Normal  Human Gingival Fibroblasts
LL-0007 , DermaLife K Medium Complete Kit
LL-0001 , FibroLife Serum-Free Medium Complete Kit
LL-0011, FibroLife S2 Medium Complete Kit
LL-0013 , TrypKit Subculturing Reagents
LM-0015, FrostaLife Cryopreservation Solution 

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