GTX00053-pro
- Brand
- GeneTex
- Application
- Functional Assay
- Storage Conditions
- For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
- Note
- For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
- Synonyms
- interleukin 1 receptor type 1
- Background
- receptor for interleukin-1; involved in inflammatory response signalling [RGD, Feb 2006]
- Conjugation
- Unconjugated
- Shipping Temperature
- 4
- Buffer
- Reconstitute with 20mM Tris and 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris (pH8.0), 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.
- Gene ID
- 25663
- Fullname
- interleukin 1 receptor type 1
- Uniport ID
- Q02955
- Application Note
- IL1R1 (Interleukin 1 Receptor Type I), also known as CD121a, is an important mediator involved in many cytokine induced immune and inflammatory responses. It belongs to the?interleukin-1 receptor?family, and is a receptor for interleukin 1 alpha (IL1A), interleukin 1 beta (IL1B), and interleukin 1 receptor antagonist (IL1RA). Besides, mouse IL1B shares 87.0% AA sequence identity with rat IL1B, suggesting the exist of cross-species activity. Thus, a binding ELISA assay was constructed to detect the association of recombinant rat IL1R1 with recombinant mouse IL1B. Briefly, IL1R1 were diluted serially in PBS with 0.1% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL1B-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-IL1R1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL1R1 with IL1B was in a dose dependent manner.
- Form
- Lyophilized powder
- Product Type
- Proteins & Peptides
- Product Link
- https://www.genetex.com/Product/Detail/Rat-IL1-Receptor-1-protein-His-tag/GTX00053-pro