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Rat Noggin protein, His and MBP tag

Catalog No.
GTX00055-pro
Pack Size
  • 10 μg
Quantity

* Free Delivery for Orders SGD$250 (excl. GST) & Above.

Brand:
Specifications
GTX00055-pro
Brand
GeneTex
Application
Functional Assay
Storage Conditions
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Synonyms
noggin
Background
secreted protein; involved in dorsal fate specification [RGD, Feb 2006]
Conjugation
Unconjugated
Shipping Temperature
4
Buffer
Reconstitute with 10mM PBS (pH7.4) to a concentration of 0.1-1.0mg/ml. Do not vortex. Lyophilized from PBS (pH7.4), 0.01% SKL, 1mM DTT, 5% Trehalose.
Gene ID
25495
Fullname
noggin
Uniport ID
Q62809
Application Note
NOG (Noggin) is a signaling molecule that plays an important role in promoting somite patterning in the developing embryo, essential for cartilage morphogenesis and joint formation. It is considered as an inhibitor in bone morphogenetic proteins (BMP) signaling, by binding with BMP4, thus a binding ELISA assay was conducted to detect the association of recombinant rat NOG with BMP4. Briefly, NOG were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to BMP4-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-NOG pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of NOG with BMP4 was in a dose dependent manner.
Form
Lyophilized powder
Product Type
Proteins & Peptides
Product Link
https://www.genetex.com/Product/Detail/Rat-Noggin-protein-His-and-MBP-tag/GTX00055-pro
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