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Rat TIMP3 protein, His tag

Catalog No.
GTX00064-pro
Pack Size
  • 10 μg
Quantity

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Brand:
Specifications
GTX00064-pro
Brand
GeneTex
Application
Functional Assay
Storage Conditions
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Synonyms
TIMP metallopeptidase inhibitor 3 , Timp-3
Background
may inhibit metalloendopeptidase activity; may play a role in induction of apoptosis [RGD, Feb 2006]
Conjugation
Unconjugated
Shipping Temperature
4
Buffer
Reconstitute with 20mM Tris and 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris (pH8.0), 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.
Gene ID
25358
Fullname
TIMP metallopeptidase inhibitor 3
Uniport ID
P48032
Application Note
Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) is an protein belongs to the tissue inhibitor of metalloproteinases family. They are inhibitors of the?matrix metalloproteinases. TIMP-3 is the only member of the TIMP family which is found exclusively in the extracellular matrix (ECM). It is regulated in a cell cycle-dependent fashion in certain cell types and may serve as a marker for terminal differentiation. Besides, Matrix Metalloproteinase 2 (MMP2) has been identified as an interactor of TIMP3, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat TIMP3 and recombinant rat MMP2. Briefly, TIMP3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to MMP2-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-TIMP3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of TIMP3 and MMP2 was in a dose dependent manner.
Form
Lyophilized powder
Product Type
Proteins & Peptides
Product Link
https://www.genetex.com/Product/Detail/Rat-TIMP3-protein-His-tag/GTX00064-pro
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