BTQ-101
- Brand
- Toyobo
- Storage Conditions
- -20℃
- Shipping Temperature
- -20℃
- Product Family
- Taq DNA polymerase
- Fidelity (3'→5' Exonuclease activity)
- 3X
- Velocity (Extension time)
- (1 min/kb)
- Amplification from crude samples
- Good/Moderate
- Reverse Transcriptase Activity
- No
- Hot-start
- No
- PCR Product Ends
- mixed (blunt &
3'-dA)
Blend Taq™ and Blend Taq™ -Plus- are highly efficient Taq-based DNA polymerases developed based on the Barns' method. This method uses a DNA polymerase which lacked 3'→5' exonuclease (proofreading) activity (e.g., Taq DNA polymerase) and a small amount of an archaeal DNA polymerase with proofreading activity. Because the proofreading activity repairs misincorporated nucleotide bases causing the termination of the polymerase reaction, PCR with a ‘mixed' enzyme solution enables highly efficient amplification. The enzyme solution of Blend Taq™ -Plus- contains anti-Taq DNA polymerase antibodies that inhibit polymerase activity, allowing for Hot Start PCR. Blend Taq™ and Blend Taq™ -Plus- generate dA overhang-ended PCR products. Therefore, the PCR products can be cloned using a standard TA-cloning method.
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