Rabbit anti-PSMB8/LMP7 Antibody, Affinity Purified
Brand:
A305-195A-T
- Brand
- Bethyl Laboratories
- Application
- IP
- Storage Conditions
- 2 - 8° C
- Host
- Rabbit
- Conjugation
- Unconjugated
- Immunogen
- between 226 and 276
- Buffer
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
- Reactivity
- Human
- Target
- PSMB8/LMP7
- Shelf Life
- 1 year from date of receipt
- Antibody Type
- Polyclonal
- Alternate Names
- ALDD, D6S216, D6S216E, JMP, LMP7, low molecular mass protein 7, low molecular weight protein 7, macropain subunit C13, multicatalytic endopeptidase complex subunit C13, NKJO, PRAAS1, protease component C13, proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7), proteasome catalytic subunit 3i, proteasome component C13, proteasome subunit beta 5i, proteasome subunit beta type-8, Proteasome subunit beta-5i, proteasome subunit Y2, proteasome-related gene 7, PSMB5i, really interesting new gene 10 protein, RING10
- Format
- Whole IgG
- Product Type
- Primary Antibodies
- Product Sub Types
- Primary Antibodies
- UNSPSC
- 12352203
- Western Blot (WB)
- Not recommended. A305-195A has not performed satisfactorily when used for WB of PSMB8 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of PSMB8.
- Immunoprecipitation (IP)
- 2 - 10 µg/mg lysate
- Target Sentence
- Proteasomes are distributed throughout eukaryotic cells and cleave peptides in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The gene PSMB8 (aka LMP7) encodes a member of the proteasome B-type family, proteasome subunit beta type-8, that is a 20S core beta subunit. Expression of PSMB8/LMP7 is induced by gamma interferon and the PSMB8 gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Nakajo syndrome, an autosomal recessive autoinflammatory disorder, is caused by mutations to PSMB8 [taken from NCBI Entrez Gene (Gene ID: 5696) and UniProtKB (Entry: P28062)].
- Product Link
- http://www.bethyl.com/product/A305-195A
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