FectoCHO® Expression System
The growing need in the biopharmaceutical market for various recombinant proteins and antibodies produced in a short time requires an efficient and flexible system. Transient expression is a commonly used process to face this demand but is unfortunately limited by transfection efficiency and inherent productivity, especially with CHO cells. To overcome this issue, we developed an advanced transient expression system, FectoCHO® Expression system, consisting in the synergistic association of a novel CHO chemically defined medium, FectoCHO® CD Expression medium and a powerful transfection reagent, FectoPRO®. FectoCHO® CD Expression medium is optimised to facilitate adaptation and cultivation of various strains of CHO cells such as CHO-K1, CHO-S and ExpiCHO™-S : extensive sequential adaptation is not required. FectoPRO®-mediated transient transfection in this chemically defined medium is optimal and therefore leads to the highest protein and antibody yields using Transient Gene Expression; with up to 4 times higher protein yields than with the competitors’ systems.
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- Brand
- Polyplus-transfection
- Application
- Protein / Antibody Production
- Previous Catalog No
- 716-01LKIT
- Cell Types
- Suspension CHO cells such as CHO-K1, CHO-S or ExpiCHO™-S
- Virus Types
- FectoCHO® CD Expression Medium
FectoPRO® Transfection reagent
FectoPRO® Expression Booster
- No. of Transfections
- 1 mL of FectoPRO® Transfection reagent is sufficient to transfect 800 ml to more than 1 liter of FectoCHO® CD Expression Medium
- Storage
- Store FectoPRO® and FectoCHO® CD Expression Medium at 5 °C ± 3°C. Do not freeze.
