1X RIPA Buffer, Biotechnology Grade
1st BASE RIPA Buffer is a ready-to-use solution designed for efficient cell lysis and protein extraction from mammalian cells and tissues. It provides quick and effective solubilisation of proteins, while preserving protein integrity and minimising interference with antigen-antibody interactions, making it ideal for a variety of downstream applications.
- Brand
- 1st BASE
- Grade
- Biotechnology Grade
- Storage Conditions
- Consists of 50 mM Tris pH 7.6, 150 mM NaCl, 1% NP-40, 0.5% Sodium Deoxycholate, 0.1% SDS at 1X concentration.
- Appearance
- Clear Liquid
- pH
- 7.4-7.8
Application
The RIPA buffer is used for lysing cells and extracting proteins from mammalian cells or soft tissue, enabling efficient cell lysis and protein extraction while minimizing effects on antigen-antibody interactions.
Composition
Ready-to-use format containing 50 mM Tris pH 7.6, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS Downstream
Applications
SDS-PAGE, Western Blotting