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ReverTra Ace™ qPCR RT Master Mix

Catalog No.
FSQ-201
ReverTra Ace qPCR RT Master Mix
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Pack Size
  • 200 reactions
Quantity

* Free Delivery for Orders SGD$250 (excl. GST) & Above.

Brand:
Specifications
FSQ-201
Brand
Toyobo
Application
qPCR
Storage Conditions
-20℃
Specificity
Excellent
Shipping Temperature
-20℃
Enzyme
ReverTra Ace™
Long cDNA synthesis
Good (< 5 kb)
Recommended PCR Enzymes
THUNDERBIRD™ series, Realtime PCR Master Mix series, KOD SYBR® qPCR Mix, KOD –Plus- series, KOD FX series, Taq-based PCR reagent
More Info
This kit includes master mix reagents for reverse transcription and for the removal of genomic DNA [DNase I treatment]. In many cases, total RNA prepared using spin-columns or acid guanidium-phenol- chloroform (AGPC) extraction methods contains small amount of genomic DNA. Any contaminating genomic DNA will be amplified along with cDNA, especially when primer pairs are designed within the same exon or from pseudogenes. Amplification from genomic DNA can result in qualitative and quantitative inaccuracies. The genomic DNA degradation and revers transcription steps can be achieved sequentially without purification or heat inactivation of DNase I.
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