100bp DNA Ladder, Ready to Use

Used for sizing and approximate quantification of wide range double-stranded DNA on agarose gel. The ladder is composed of eleven individual DNA fragments (in base pairs): 1500, 1000, 900, 800, 700, 600, 500, 400, 300, 200 and 100. It contains two reference bands of 1500 and 500bp for easy orientation. Orange G and xylene cyanol FF are used as tracking dyes.

Storage Buffer

10mM Tris-HCl (pH 8.0)

1mM EDTA

Protocol for Loading

• Load 5µl of the solution into a 5mm agarose gel lane.
• It is recommended to load 1μl (0.1μg) of DNA ladder per 1mm of gel lane.

Recommendations

• Do not heat solution prior to loading.
• Adjust sample concentration to be approximately equal to the amount of DNA in the nearest band of the ladder for quantification purpose.
• Visualize DNA by staining with DNA stain (C/No: BIO-5170-1ml).