HSTTX-111
- Brand
- Toyobo
- Storage Conditions
- -20℃
- Specificity
- Excellent
- Shipping Temperature
- -20℃
- Reverse Transcriptase Activity
- Yes
- Hot-start
- Yes
- Assay Type
- Probe
- One-step RT-PCR
- Yes
- Passive Reference
- Yes
- Compatible with Glass Capillary
- Yes
- Efficiency
- Excellent
- Long Target Amplification
- Good
- GC-rich Target
- Good
Hot Start TTx (RNA) Kit is RT-PCR reagent based on our original polymerase, TTx DNA Polymerase. TTx DNA polymerase exhibits reverse transcriptase activity in the presence of Mn2+ ions. This system allows for 1-step real-time PCR, including reverse transcription and PCR steps. TTx DNA Polymerase has higher amplification efficiency than Taq DNA Polymerase, which is a general-purpose enzyme, and has reverse transcription activity, enabling amplification from a crude sample containing PCR inhibitors with high efficiency from both DNA and RNA. In addition, TTx DNA Polymerase has a 5 '- 3' exonuclease activity, so it can be used for real-time PCR using probe assays such as TaqMan® assay. This enzyme contains neutralizing antibodies, thus allowing for Hot start PCR.
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