KOD Dash
Catalog No.
LDP-101
KOD Dash
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Brand:
LDP-101
- Brand
- Toyobo
- Storage Conditions
- -20℃
- Shipping Temperature
- -20℃
- Product Family
- KOD DNA polymerase
- Fidelity (3'→5' Exonuclease activity)
- 3X
- Velocity (Extension time)
- (≤0.5 min/kb)
- Amplification from crude samples
- Good/Moderate
- Reverse Transcriptase Activity
- No
- Hot-start
- No
- PCR Product Ends
- mixed (blunt &
3'-dA)
KOD Dash is a highly efficient DNA polymerase mixture developed based on the Barns' method. This method uses a DNA polymerase which lacked a 3'→5' exonuclease (proofreading) activity and a small amount of an archaeal DNA polymerase with proofreading activity. In the reagent, the 3'→5' exonuclease activity-minus mutant of KOD DNA polymerase and KOD DNA polymerase are used. Because the proofreading activity repairs misincorporated nucleotide bases causing the termination of a polymerase reaction, PCR with this mixed enzyme solution enables highly efficient amplification. KOD Dash generates dA overhang-ended PCR products. Therefore, the PCR products can be cloned using a standard TA cloning method.
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