KME-101
- Brand
- Toyobo
- Storage Conditions
- -20℃
- Shipping Temperature
- -20℃
- Product Family
- KOD DNA polymerase
- Fidelity (3'→5' Exonuclease activity)
- 11X
- Velocity (Extension time)
- (≤15 sec/kb)
- Amplification from crude samples
- Excellent
- Reverse Transcriptase Activity
- No
- Hot-start
- Yes
- PCR Product Ends
- blunt
KOD -Multi & Epi-™ is a high-fidelity PCR enzyme based on genetically modified KOD DNA polymerase(UKOD). This modified enzyme enables amplification from templates containing uracils (U) or using primers containing inosines (I) and uracils (U). Furthermore, addition of the Elongation Accelerator significantly reduces amplification bias during PCR. KOD -Multi & Epi-™ can be applied to various purposes such as a) multiplex PCR, b) bisulfite PCR in epigenetics research, and c) metagenomics research. This enzyme is also applicable for the preparation of DNA fragments for next-generation sequencing and capillary sequencing via cloning because the enzyme exhibits about 11-fold higher PCR fidelity.
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