MangoMix, 2x

MangoTaq™ DNA Polymerase is a formulation of Taq DNA Polymerase which offers consistent results across a wide range of DNA templates. MangoTaq DNA Polymerase possesses 5’- 3’ exonuclease activity and leaves an ‘A’ overhang such that the PCR product is suitable for effective integration into TA cloning vectors. For high-throughput applications, MangoTaq and the colored reaction buffer make an ideal choice, since this combination enables the user to load directly on a gel, and facilitates easy recognition. The two reaction buffers supplied are: 5x Colored reaction buffer and 5x Colorless reaction buffer. The colored reaction buffer contains red and orange dyes, which separate during electrophoresis and provide quick reference points for monitoring the mobility of the DNA samples in the gel. The colored reaction buffer can be loaded directly onto an agarose gel for analysis, without the need for separate gel-loading buffer. The presence of the dyes has no effect on most routine enzymatic manipulations. MangoMix™ is a complete ready-to-use 2x pre-optimized reaction mix containing MangoTaq DNA Polymerase, Mg2+, dNTPs, red and orange reference dyes. MangoMix enables users to perform PCR assays of most common genomic and cDNA templates, simply requiring the addition of water, template and primers to perform the assays. MangoMix dramatically reduces the time required to set up reactions, thereby minimizing the risk of contamination.