MyFi DNA Polymerase

MyFi™ is a novel, antibody-mediated, hot-start enzyme with unique properties that offers 3.5x higher fidelity than native Taq and enhanced sensitivity and specificity, making MyFi a superior choice for cloning. The polymerase is supplied with MyFi Buffer, a highly optimized proprietary formulation, containing ultra-pure dNTPs, MgCl2 and enhancers, specifically formulated and validated to enhance your results. MyFi is ideally suited for difficult PCR amplification of targets with variable lengths up to 10 kb, for example, amplification of cDNA libraries, complex genomic fragments, targets with high GC-content and low-copy assays which require both high processivity and higher fidelity. MyFi has the added convenience of room temperature reaction assembly, to avoid non-specific amplification and primer-dimer formation. MyFi generates PCR products with 3’-A overhangs, perfect for TA cloning. MyFi Mix is a ready-to-use 2x mix, containing all the reagents (including enhancers and stabilizers) necessary for trouble-free PCR reaction set-up. The unique MyFi Mix, supplied in a convenient single tube, reduces the number of pipetting steps and improves efficiency, throughput and reproducibility.