Thermo T7 RNA Polymerase 《TT7》
Catalog No.
TRL-201
Thermo T7 RNA Polymerase (TT7)
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Brand:
TRL-201
- Brand
- Toyobo
- Application
- RNA polymerase
- Storage Conditions
- -20℃
- Shipping Temperature
- -20℃
- Assay Condition
- 40mM Tris-HCl(pH8.0), 50mM NaCl, 8mM MgCl2, 5mM DTT, 400µM rNTPs, 400µM [3H]-UTP(30cpm/pmoles), 20µg/mL T7 DNA, 50µg/mL BSA, 100µL reaction volume. 37℃, 10min.
Derived from Escherichia coli carrying the plasmid that encodes the gene of phage T7 RNA polymerase. One unit of enzyme is defined as the amount of enzyme that will incorporate 1 nmole of labeled nucleotide into acid insoluble material in 1 hour at 37℃ under standard assay conditions as described below.
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