Hi-yield T7 in vitro transcription reagent (N1-Me-pUTP)

Hi-yield T7 in vitro transcription reagent kit is designed for in vitro transcription of RNA using T7 RNA polymerase. The kit is capable of synthesizing high yield RNA transcripts using linear double-stranded DNA containing T7 promoter as template and NTPs as substrate. The kit is suitable for incorporation of modified nucleotides to obtain biotin labeled, dye labeled and radiolabeled RNA. The kit also can be applied in synthesis of co-transcriptionally capping mRNAs with cap analogs. The kit contains modified nucleotides N1-Me-pUTP as alternative. Each standard reaction yields up to 150-200 μg of RNA from 1μg DNA template. Reaction size can be scaled up to yield milligram-level RNA as needed. RNA synthesized from the kit is suitable for many applications including RNA structure and function studies, ribozyme biochemistry, RNase protection assays and hybridization-based blots, anti-sense RNA and RNAi experiment. RNA synthesized from the kit also is suitable for producing enzymatic production of capped RNA by vaccinia capping enzyme (HBP000606) and 2’-O-Methyltransferase (HBP000701) and being tailed up with Poly(A) polymerase (HBP000801) to improve the stability and translational competence of RNA used for in vitro translation, transfection, and microinjection.