PNGase F-Fast

PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F is an amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins. It is very important for effective process control to obtain the accurate distribution of N-glycans in a short time. Generally, it takes several hours to hydrolyze and release the N-glycans of the antibody with PNGase F, and then carry out glycan derivatization and HPLC or mass spectrometry analysis. Especially, incomplete deglycosylation can lead to biased results, which will not represent the correct composition of the therapeutic antibody. PNGase F-Fast is an improved reagent, which can release the N-glycans of therapeutic antibody in a few minutes. All N-glycans can be released quickly without bias, and are ready for downstream chromatography or mass spectrometry analysis, so as to determine the glycoprofile of antibodies quickly.