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SensiFAST HRM Kit

Catalog No.
BIO-32005
High Resolution Melt curve (HRM) analysis characterizes nucleic acid samples based on their dissociation behavior. It combines the principle of intercalating dyes, melt curve analyses and the application of specific statistical analyses. HRM uses the fundamental property of the separation of the two strands of DNA with heat (melting), and the monitoring of this melting with a fluorescent dye. The SensiFAST HRM Kit has been developed for fast, highly reproducible High Resolution Melt (HRM) analysis and has been validated on commonly used real-time instruments. A combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, ensures that the SensiFAST HRM Kit delivers fast, highly-specific and ultra-sensitive HRM analysis. Main applications of HRM include Single Nucleotide Polymorphisms (SNPs) genotyping (fig. 1), epigenetics (DNA methylation analysis), zygosity testing (DNA mapping and DNA fingerprinting) and gene scanning (search for the presence of unknown variations in PCR amplicons). For ease-of-use and added convenience, SensiFAST HRM is provided as a 2x master mix containing all the components necessary for qPCR, including the EvaGreen® dye, dNTPs, stabilisers and enhancers. As a ready-to-use premix, only primers and template need to be added.
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Pack Size
  • 500 Reactions
  • 2000 Reactions
Quantity

* Free Delivery for Orders SGD$250 (excl. GST) & Above.

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