Rabbit anti-ARNT Antibody, Affinity Purified
Brand:
A302-764A-T
- Brand
- Bethyl Laboratories
- Application
- IHC
IP
- Storage Conditions
- 2 - 8° C
- Host
- Rabbit
- Conjugation
- Unconjugated
- Immunogen
- between 1 and 50
- Buffer
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
- Reactivity
- Human
- Target
- ARNT
- Shelf Life
- 1 year from date of receipt
- Antibody Type
- Polyclonal
- Alternate Names
- ARNT protein, aryl hydrocarbon receptor nuclear translocator, bHLHe2, class E basic helix-loop-helix protein 2, dioxin receptor, nuclear translocator, HIF1B, HIF1BETA, HIF-1beta, HIF-1-beta, HIF1-beta, hypoxia-inducible factor 1, beta subunit, Hypoxia-inducible factor 1-beta, TANGO
- Format
- Whole IgG
- Presumed Reactivity
- Based on 100% sequence identity, this antibody is predicted to react with Bovine
- Product Type
- Primary Antibodies
- Product Sub Types
- Primary Antibodies
- UNSPSC
- 12352203
- Western Blot (WB)
- Not recommended. A302-764A has not performed satisfactorily when used for WB of ARNT in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of ARNT.
- Immunoprecipitation (IP)
- 2 - 5 µg/mg lysate
- Immunohistochemistry (IHC)
- 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
- Target Sentence
- Aryl hydrocarbon receptor nuclear translocator (ARNT) is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. ARNT forms a complex with the ligand-bound Ah receptor, and is required for receptor function. ARNT has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1). A t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia [taken from NCBI Entrez Gene (GeneID: 405)].
- Product Link
- http://www.bethyl.com/product/A302-764A
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