Rabbit anti-Bcl-2 Antibody, Affinity Purified
Brand:
A303-675A-T
- Brand
- Bethyl Laboratories
- Application
- IHC
IP
- Storage Conditions
- 2 - 8° C
- Host
- Rabbit
- Conjugation
- Unconjugated
- Immunogen
- between 50 and 100
- Buffer
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
- Reactivity
- Human
- Target
- Bcl-2
- Shelf Life
- 1 year from date of receipt
- Antibody Type
- Polyclonal
- Alternate Names
- apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, Bcl-2, PPP1R50, protein phosphatase 1, regulatory subunit 50
- Format
- Whole IgG
- Product Type
- Primary Antibodies
- Product Sub Types
- Primary Antibodies
- UNSPSC
- 12352203
- Western Blot (WB)
- Not recommended. A303-675A has not performed satisfactorily when used for WB of Bcl-2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Bcl-2.
- Immunoprecipitation (IP)
- 2 - 10 µg/mg lysate
- Immunohistochemistry (IHC)
- 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
- Target Sentence
- B-cell CLL/lymphoma 2 (Bcl-2) is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of Bcl-2, such as in the case of translocation of Bcl-2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma [taken from NCBI Entrez Gene (Gene ID: 596)].
- Product Link
- http://www.bethyl.com/product/A303-675A
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