A302-270A-T
- Brand
- Bethyl Laboratories
- Application
- IHC
IP
- Storage Conditions
- 2 - 8° C
- Host
- Rabbit
- Conjugation
- Unconjugated
- Immunogen
- Between 1784 and 1834
- Buffer
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
- Reactivity
- Human
- Target
- BTBD12
- Shelf Life
- 1 year from date of receipt
- Antibody Type
- Polyclonal
- Alternate Names
- BTB/POZ domain-containing protein 12, BTBD12, FANCP, MUS312, structure-specific endonuclease subunit SLX4
- Format
- Whole IgG
- Product Type
- Primary Antibodies
- Product Sub Types
- Primary Antibodies
- UNSPSC
- 12352203
- Western Blot (WB)
- Not recommended. A302-270A has not performed satisfactorily when used for WB of BTBD12 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of BTBD12.
- Immunoprecipitation (IP)
- 10 µg/mg lysate
- Immunohistochemistry (IHC)
- 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
- Target Sentence
- BTBD12 (BTBD12 domain-containing protein 12) has been identified as the human ortholog of the yeast Slx4p and drosophila MUS312 DNA repair factors. BTBD12 is an ATM/ATR checkpoint substrate that functions as part of a Holliday junction resolvase and is important for DNA interstrand crosslink repair.
- Product Link
- http://www.bethyl.com/product/A302-270A