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Rabbit anti-Bub3 Antibody, Affinity Purified

Catalog No.
A300-365A-M
Pack Size
  • 20 µl (2 blots)
  • 100 µl (10 blots)
  • 100 µl (1000 µg/ml)
Quantity
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Brand:
Specifications
A300-365A-M
Brand
Bethyl Laboratories
Application
IHC
WB
Storage Conditions
2 - 8° C
Host
Rabbit
Conjugation
Unconjugated
Immunogen
Between 275 and 325
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Reactivity
Human
Target
Bub3
Shelf Life
1 year from date of receipt
Antibody Type
Polyclonal
Alternate Names
BUB3 budding uninhibited by benzimidazoles 3 homolog, BUB3L, budding uninhibited by benomyl, budding uninhibited by benzimidazoles 3 homolog, hBUB3, mitotic checkpoint component, mitotic checkpoint protein BUB3, testicular tissue protein Li 27
Format
Whole IgG
Presumed Reactivity
Based on 100% sequence identity, this antibody is predicted to react with Mouse, Bovine and Orangutan
Product Type
Primary Antibodies
Product Sub Types
Primary Antibodies
UNSPSC
12352203
Western Blot (WB)
1:5,000 - 1:15,000
Immunohistochemistry (IHC)
1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Sentence
Bub3 is an adaptor protein and component of the spindle checkpoint machinery. Bub3 and other spindle checkpoint proteins ensure that segregation does not proceed until all replicated chromatids are associated with a functional bipolar spindle. Bub3 forms a constitutive complex with Bub1. Bub3 has been demonstrated to also function as a transcriptional repressor in association with Cdc20 and histone deacetylases.
Product Link
http://www.bethyl.com/product/A300-365A
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