Rabbit anti-CHCHD4 Antibody, Affinity Purified
Brand:
A305-810A-M
- Brand
- Bethyl Laboratories
- Application
- IP
- Storage Conditions
- 2 - 8° C
- Host
- Rabbit
- Conjugation
- Unconjugated
- Immunogen
- between 92 and 142
- Buffer
- Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
- Reactivity
- Human
- Target
- CHCHD4
- Shelf Life
- 1 year from date of receipt
- Antibody Type
- Polyclonal
- Alternate Names
- coiled-coil-helix-coiled-coil-helix domain-containing protein 4, MIA40, mitochondrial intermembrane space import and assembly protein 40, TIMM40, translocase of inner mitochondrial membrane 40 homolog
- Format
- Whole IgG
- Product Type
- Primary Antibodies
- Product Sub Types
- Primary Antibodies
- UNSPSC
- 12352203
- Western Blot (WB)
- Not recommended. Use rabbit anti-CHCHD4 antibody A305-811A-T.
- Immunoprecipitation (IP)
- 15-20 µl/mg lysate
- Target Sentence
- CHCHD4 functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17, COX19 and MICU1. Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS. Reduced CHCHD4/MIA40 is then reoxidized by GFER/ERV1 via a disulfide relay system. Mediates formation of disulfide bond in MICU1 in the IMS, promoting formation of the MICU1-MICU2 heterodimer that regulates mitochondrial calcium uptake. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8N4Q1].
- Product Link
- http://www.bethyl.com/product/A305-810A-T