A300-256A-M
- Brand
- Bethyl Laboratories
- Application
- IHC
IP
WB
- Storage Conditions
- 2 - 8° C
- Host
- Rabbit
- Conjugation
- Unconjugated
- Immunogen
- between 250 and 300
- Buffer
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
- Reactivity
- Human
- Target
- Fen1
- Shelf Life
- 1 year from date of receipt
- Antibody Type
- Polyclonal
- Alternate Names
- DNase IV, FEN-1, flap endonuclease 1, Flap structure-specific endonuclease 1, hFEN-1, Maturation factor 1, maturation factor-1, MF1, RAD2
- Format
- Whole IgG
- Presumed Reactivity
- Based on 100% sequence identity, this antibody is predicted to react with Sheep and Bovine
- Product Type
- Primary Antibodies
- Product Sub Types
- Primary Antibodies
- UNSPSC
- 12352203
- Western Blot (WB)
- 1:2,000 - 1:10,000
- Immunoprecipitation (IP)
- 2 - 10 µg/mg lysate
- Immunohistochemistry (IHC)
- 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. 16-18 hour incubation is recommended.
- Target Sentence
- Flap endonuclease 1 (Fen1) is a member of the XPG/RAD2 endonuclease family. Fen1 functions primarily as an endonuclease that removes 5’-overhangs during base-excision DNA repair and lagging strand DNA synthesis. For these processes Fen1 interacts with PCNA and the 9-1-1 DNA repair complex. Fen1 also possesses 5’ to 3’ exonuclease activity as well as RNase H activity.
- Product Link
- http://www.bethyl.com/product/A300-256A