Rabbit anti-FIH-1 Antibody, Affinity Purified
Brand:
A303-550A-T
- Brand
- Bethyl Laboratories
- Application
- IHC
IP
- Storage Conditions
- 2 - 8° C
- Host
- Rabbit
- Conjugation
- Unconjugated
- Immunogen
- between 300 and 349
- Buffer
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
- Reactivity
- Human
- Target
- FIH-1
- Shelf Life
- 1 year from date of receipt
- Antibody Type
- Polyclonal
- Alternate Names
- factor inhibiting HIF1, factor inhibiting HIF-1, FIH1, FIH-1, hypoxia inducible factor 1 alpha subunit inhibitor, hypoxia-inducible factor 1-alpha inhibitor, hypoxia-inducible factor asparagine hydroxylase, peptide-aspartate beta-dioxygenase
- Format
- Whole IgG
- Presumed Reactivity
- Based on 100% sequence identity, this antibody is predicted to react with Mouse and Zebrafish
- Product Type
- Primary Antibodies
- Product Sub Types
- Primary Antibodies
- UNSPSC
- 12352203
- Western Blot (WB)
- Not recommended
- Immunoprecipitation (IP)
- 2 - 10 µg/mg lysate
- Immunohistochemistry (IHC)
- 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
- Target Sentence
- Factor inhibiting HIF-1 (FIH-1) hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). FIH-1 functions as an oxygen sensor, and under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. FIH-1 is involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9NWT6].
- Product Link
- http://www.bethyl.com/product/A303-550A
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