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Rabbit anti-GTF2A1/TFIIA Antibody, Affinity Purified

Catalog No.
A302-777A-T
Pack Size
  • 100 µl (1000 µg/ml)
  • 10 µl (1000 µg/ml)
Quantity

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Brand:
Specifications
A302-777A-T
Brand
Bethyl Laboratories
Application
IHC
IP
Storage Conditions
2 - 8° C
Host
Rabbit
Conjugation
Unconjugated
Immunogen
between 100 and 150
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Reactivity
Human, Mouse
Target
GTF2A1/TFIIA
Shelf Life
1 year from date of receipt
Antibody Type
Polyclonal
Alternate Names
general transcription factor IIA 1, General transcription factor IIA subunit 1, general transcription factor IIA, 1, 19/37kDa, glucose regulated protein, 58kD pseudogene, TF2A1, TFIIA, TFIIA alpha, p55, TFIIA alpha/beta subunits, TFIIA-42, TFIIAL, transcription initiation factor IIA subunit 1, transcription initiation factor TFIIA 42 kDa subunit
Format
Whole IgG
Presumed Reactivity
Based on 100% sequence identity, this antibody is predicted to react with Rat and Orangutan
Product Type
Primary Antibodies
Product Sub Types
Primary Antibodies
UNSPSC
12352203
Western Blot (WB)
Not recommended. A302-777A has not performed satisfactorily when used for WB of TFIIA in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of TFIIA.
Immunoprecipitation (IP)
2 - 5 µg/mg lysate
Immunohistochemistry (IHC)
1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Sentence
GTF2A1/TFIIA (general transcription factor IIA subunit 1) is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. gTF2A1/TFIIA in a complex with TBP mediates transcriptional activity [taken from the Universal Protein Resource (UniProt) ww.uniprot.org/uniprot/P52655].
Product Link
http://www.bethyl.com/product/A302-777A
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