Rabbit anti-GTF2A1/TFIIA Antibody, Affinity Purified
A302-777A-T
- Brand
- Bethyl Laboratories
- Application
- IHC
IP
- Storage Conditions
- 2 - 8° C
- Host
- Rabbit
- Conjugation
- Unconjugated
- Immunogen
- between 100 and 150
- Buffer
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
- Reactivity
- Human, Mouse
- Target
- GTF2A1/TFIIA
- Shelf Life
- 1 year from date of receipt
- Antibody Type
- Polyclonal
- Alternate Names
- general transcription factor IIA 1, General transcription factor IIA subunit 1, general transcription factor IIA, 1, 19/37kDa, glucose regulated protein, 58kD pseudogene, TF2A1, TFIIA, TFIIA alpha, p55, TFIIA alpha/beta subunits, TFIIA-42, TFIIAL, transcription initiation factor IIA subunit 1, transcription initiation factor TFIIA 42 kDa subunit
- Format
- Whole IgG
- Presumed Reactivity
- Based on 100% sequence identity, this antibody is predicted to react with Rat and Orangutan
- Product Type
- Primary Antibodies
- Product Sub Types
- Primary Antibodies
- UNSPSC
- 12352203
- Western Blot (WB)
- Not recommended. A302-777A has not performed satisfactorily when used for WB of TFIIA in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of TFIIA.
- Immunoprecipitation (IP)
- 2 - 5 µg/mg lysate
- Immunohistochemistry (IHC)
- 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
- Target Sentence
- GTF2A1/TFIIA (general transcription factor IIA subunit 1) is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. gTF2A1/TFIIA in a complex with TBP mediates transcriptional activity [taken from the Universal Protein Resource (UniProt) ww.uniprot.org/uniprot/P52655].
- Product Link
- http://www.bethyl.com/product/A302-777A