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Rabbit anti-Importin 4 Antibody, Affinity Purified

Catalog No.
A302-724A-T
Pack Size
  • 100 µl (1000 µg/ml)
  • 10 µl (1000 µg/ml)
Quantity
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Brand:
Specifications
A302-724A-T
Brand
Bethyl Laboratories
Application
IHC
IP
Storage Conditions
2 - 8° C
Host
Rabbit
Conjugation
Unconjugated
Immunogen
between 350 and 400
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Reactivity
Human
Target
Importin 4
Shelf Life
1 year from date of receipt
Antibody Type
Polyclonal
Alternate Names
Imp4, imp4b, importin-4, importin-4b, ran-binding protein 4, ranBP4
Format
Whole IgG
Product Type
Primary Antibodies
Product Sub Types
Primary Antibodies
UNSPSC
12352203
Western Blot (WB)
Not recommended. A302-724A has not performed satisfactorily when used for WB of Importin 4 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Importin 4.
Immunoprecipitation (IP)
5 - 15 µg/mg lysate
Immunohistochemistry (IHC)
1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Sentence
Importin 4 mediates the nuclear import of RPS3A. In vitro, it mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Additionally, along with importin 7, importin 4 has been shown to be involved in nuclear import of HIF-1alpha [taken from the Universal Protein Resource (UniProt) ww.uniprot.org/uniprot/Q8TEX9].
Product Link
http://www.bethyl.com/product/A302-724A
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