Rabbit anti-LIN28B Antibody, Affinity Purified
Brand:
A303-589A-T
- Brand
- Bethyl Laboratories
- Application
- IHC
IP
- Storage Conditions
- 2 - 8° C
- Host
- Rabbit
- Conjugation
- Unconjugated
- Immunogen
- between 200 and 250
- Buffer
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
- Reactivity
- Human
- Target
- LIN28B
- Shelf Life
- 1 year from date of receipt
- Antibody Type
- Polyclonal
- Alternate Names
- CSDD2, Lin-28.2, lin-28B, protein lin-28 homolog B
- Format
- Whole IgG
- Product Type
- Primary Antibodies
- Product Sub Types
- Primary Antibodies
- UNSPSC
- 12352203
- Western Blot (WB)
- Not recommended
- Immunoprecipitation (IP)
- 2 - 10 µg/mg lysate
- Immunohistochemistry (IHC)
- 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
- Target Sentence
- Lin-28 homolog B (LIN28B) acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. LIN28B acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. LIN28B specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. It also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q6ZN17].
- Product Link
- http://www.bethyl.com/product/A303-589A
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