Rabbit anti-MCAK Antibody, Affinity Purified
Brand:
A300-921A-M
- Brand
- Bethyl Laboratories
- Application
- IHC
IP
WB
- Storage Conditions
- 2 - 8° C
- Host
- Rabbit
- Conjugation
- Unconjugated
- Immunogen
- between 50 and 100
- Buffer
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
- Reactivity
- Mouse
- Target
- MCAK
- Shelf Life
- 1 year from date of receipt
- Antibody Type
- Polyclonal
- Alternate Names
- 4930402F02Rik, ESTM5, kinesin-like 6, kinesin-like protein KIF2C, Knsl6, MCAK, mitotic centromere-associated kinesin, X83316
- Format
- Whole IgG
- Presumed Reactivity
- Based on 100% sequence identity, this antibody is predicted to react with Rat
- Product Type
- Primary Antibodies
- Product Sub Types
- Primary Antibodies
- UNSPSC
- 12352203
- Western Blot (WB)
- 1:2,000 - 1:10,000
- Immunoprecipitation (IP)
- 2 - 5 µg/mg lysate. A300-921A is less efficient at IP than is A300-922A.
- Immunohistochemistry (IHC)
- 1:500 - 1:2,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
- Target Sentence
- Kinesin activity has been linked to various cellular functions such as vesicle transport, mitotic spindle formation, chromosome segregation, chromosome congression, and cytokinesis. Structurally, all kinesins contain a motor domain with microtubule and nucleotide binding sites that utilize ATP to target cargo along microtubule filaments. MCAK (Mitotic Centromere-Associated Kinesin) is the founding member of kinesin-13 proteins. There are 3 independent genes coding for kinesin-13 proteins kif2a, kif2b, and kif2c (MCAK). Instead of functioning as a translocator of microtubules MCAK is a depolymerizer that is primarily responsible for releasing improper microtubule-kinetochore attachments during cell division.
- Product Link
- http://www.bethyl.com/product/A300-921A
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