Rabbit anti-MDC1 Antibody, Affinity Purified
Brand:
A300-053A-M
- Brand
- Bethyl Laboratories
- Application
- IHC
IP
WB
- Storage Conditions
- 2 - 8° C
- Host
- Rabbit
- Conjugation
- Unconjugated
- Immunogen
- between 1375 and 1425
- Buffer
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
- Reactivity
- Human
- Target
- MDC1
- Shelf Life
- 1 year from date of receipt
- Antibody Type
- Polyclonal
- Alternate Names
- homologue to Drosophila photoreceptor protein calphotin, mediator of DNA damage checkpoint protein 1, NFBD1, nuclear factor with BRCT domains 1
- Format
- Whole IgG
- Presumed Reactivity
- Based on 100% sequence identity, this antibody is predicted to react with Chimpanzee
- Product Type
- Primary Antibodies
- Product Sub Types
- Primary Antibodies
- UNSPSC
- 12352203
- Western Blot (WB)
- 1:5,000 - 1:25,000
- Immunoprecipitation (IP)
- 2 - 5 µg/mg lysate
- Immunohistochemistry (IHC)
- 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
- Target Sentence
- Mediator of DNA damage checkpoint protein 1 (Mdc1) is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. It may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. It is also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q5PSV9].
- Product Link
- http://www.bethyl.com/product/A300-053A
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