Rabbit anti-Mdc1/Nfbd1 Antibody, Affinity Purified
Brand:
A304-843A-T
- Brand
- Bethyl Laboratories
- Application
- IP
- Storage Conditions
- 2 - 8° C
- Host
- Rabbit
- Conjugation
- Unconjugated
- Immunogen
- between 900 and 950
- Buffer
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
- Reactivity
- Mouse
- Target
- Mdc1/Nfbd1
- Shelf Life
- 1 year from date of receipt
- Antibody Type
- Polyclonal
- Alternate Names
- 6820401C03, AA413496, mediation of DNA damage checkpoint 1, mediator of DNA damage checkpoint protein 1, mKIAA0170, Nfbd1
- Format
- Whole IgG
- Product Type
- Primary Antibodies
- Product Sub Types
- Primary Antibodies
- UNSPSC
- 12352203
- Western Blot (WB)
- Not recommended. A304-843A has not performed satisfactorily when used for WB of Mdc1/Nfbd1 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Mdc1/Nfbd1.
- Immunoprecipitation (IP)
- 2 - 10 µg/mg lysate
- Target Sentence
- Mediator of DNA damage checkpoint protein 1 (Mdc1) is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. It may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. It is also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q5PSV9].
- Product Link
- http://www.bethyl.com/product/A304-843A
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