Rabbit anti-MGEA5 Antibody, Affinity Purified
Brand:
A304-345A-T
- Brand
- Bethyl Laboratories
- Application
- IHC
IP
- Storage Conditions
- 2 - 8° C
- Host
- Rabbit
- Conjugation
- Unconjugated
- Immunogen
- Between 500 and 550
- Buffer
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
- Reactivity
- Human
- Target
- MGEA5
- Shelf Life
- 1 year from date of receipt
- Antibody Type
- Polyclonal
- Alternate Names
- beta-hexosaminidase, beta-N-acetylglucosaminidase, beta-N-acetylhexosaminidase, bifunctional protein NCOAT, hyaluronidase in meningioma, MEA5, meningioma expressed antigen 5 (hyaluronidase), meningioma-expressed antigen 5, MGEA5, N-acetyl-beta-D-glucosaminidase, N-acetyl-beta-glucosaminidase, NCOAT, nuclear cytoplasmic O-GlcNAcase and acetyltransferase, OGA, protein O-GlcNAcase
- Format
- Whole IgG
- Product Type
- Primary Antibodies
- Product Sub Types
- Primary Antibodies
- UNSPSC
- 12352203
- Western Blot (WB)
- Not recommended. A304-345A has not performed satisfactorily when used for WB of MGEA5 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of MGEA5.
- Immunoprecipitation (IP)
- 2 - 10 µg/mg lysate
- Immunohistochemistry (IHC)
- 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
- Target Sentence
- Meningioma-expressed antigen 5 (MGEA5) cleaves GlcNAc but not GalNAc from glycopeptides. MGEA5 can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc.MGEA5 possesses hyaluronidase activity. Acetylates 'Lys-8' of histone H4 and 'Lys-14' of histone H3 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O60502].
- Product Link
- http://www.bethyl.com/product/A304-345A
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