Rabbit anti-NELFE Antibody, Affinity Purified
Brand:
A301-914A-T
- Brand
- Bethyl Laboratories
- Application
- IHC
IP
- Storage Conditions
- 2 - 8° C
- Host
- Rabbit
- Conjugation
- Unconjugated
- Immunogen
- between 330 and 380
- Buffer
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
- Reactivity
- Human
- Target
- NELFE
- Shelf Life
- 1 year from date of receipt
- Antibody Type
- Polyclonal
- Alternate Names
- D6S45, major histocompatibility complex gene RD, negative elongation factor E, negative elongation factor polypeptide E, NELF-E, nuclear protein, RD, RD RNA-binding protein, RDBP, RDP, RNA-binding protein RD
- Format
- Whole IgG
- Presumed Reactivity
- Based on 100% sequence identity, this antibody is predicted to react with Bovine
- Product Type
- Primary Antibodies
- Product Sub Types
- Primary Antibodies
- UNSPSC
- 12352203
- Western Blot (WB)
- Not recommended. A301-914A has not performed satisfactorily when used for WB of NELFE in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of NELFE.
- Immunoprecipitation (IP)
- 2 - 5 µg/mg lysate
- Immunohistochemistry (IHC)
- 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
- Target Sentence
- NELFE (negative elongation factor E) is one of four subunits that make up the NELF transcription elongation factor. The four subunits of NELF are NELF A (WHSC2), NELFB, either NELFC or NELFD, and NELFE. NELF, along with DRB sensitivity-inducing factor (DSIF), functions to cause transcriptional pausing of RNA polymerase II. NELF-E is an RNA-binding protein. The RNA binding activity of NELF-E is critical to the function of NELF.
- Product Link
- http://www.bethyl.com/product/A301-914A
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