Rabbit anti-Phospho Histone H3 (S10) Antibody, Affinity Purified
A301-844A
- Brand
- Bethyl Laboratories
- Application
- F
ICC-IF
IHC
WB
- Storage Conditions
- 2 - 8° C
- Host
- Rabbit
- Conjugation
- Unconjugated
- Immunogen
- surrounding serine 10
- Buffer
- Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
- Reactivity
- Human, Mouse
- Target
- Histone H3, Phospho (S10)
- Shelf Life
- 1 year from date of receipt
- Antibody Type
- Polyclonal
- Alternate Names
- H3 histone family, member A, H3/A, H3FA, histone 1, H3a, histone cluster 1, H3a, histone H3.1, histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l
- Format
- Whole IgG
- Presumed Reactivity
- Based on 100% sequence identity, this antibody is predicted to react with Rat, D. melanogaster, Zebrafish, X. laevis, X. tropicalis, Chicken, Bovine, Rabbit, Pig and Orangutan
- Product Type
- Primary Antibodies
- Product Sub Types
- Primary Antibodies
- UNSPSC
- 12352203
- Western Blot (WB)
- 1:5,000 - 1:10,000
- Immunohistochemistry (IHC)
- 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
- Immunocytochemistry-Immunofluoresence (ICC-IF)
- 1:2,000 - 1:10,000. Formaldehyde fixation and permeabilization with Triton-X 100 is recommended. Acetone fixation may adversely effect staining.
- Flow Cytometry (F)
- 0.4 µg per 1 X 10^6 cells in a 150 µl volume
- Target Sentence
- Histone H3 is one of four histone proteins that assemble into an octamer to form the nucleosome core and serve to spool and compact DNA into chromatin. Histones are subject to a complex set of post-translational modifications (acetylation, methylation, phosphorylation, ubiquitination, sumoylation, citrullination, and ADP ribosylation) that are important to the regulation of gene transcription, DNA replication, and DNA repair.
- Product Link
- http://www.bethyl.com/product/A301-844A