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Rabbit anti-Phospho Histone H3 (S10) Antibody, Affinity Purified

Catalog No.
A301-844A
Pack Size
  • 100 µl (200 µg/ml)
Quantity

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Brand:
Specifications
A301-844A
Brand
Bethyl Laboratories
Application
F
ICC-IF
IHC
WB
Storage Conditions
2 - 8° C
Host
Rabbit
Conjugation
Unconjugated
Immunogen
surrounding serine 10
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Reactivity
Human, Mouse
Target
Histone H3, Phospho (S10)
Shelf Life
1 year from date of receipt
Antibody Type
Polyclonal
Alternate Names
H3 histone family, member A, H3/A, H3FA, histone 1, H3a, histone cluster 1, H3a, histone H3.1, histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l
Format
Whole IgG
Presumed Reactivity
Based on 100% sequence identity, this antibody is predicted to react with Rat, D. melanogaster, Zebrafish, X. laevis, X. tropicalis, Chicken, Bovine, Rabbit, Pig and Orangutan
Product Type
Primary Antibodies
Product Sub Types
Primary Antibodies
UNSPSC
12352203
Western Blot (WB)
1:5,000 - 1:10,000
Immunohistochemistry (IHC)
1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunocytochemistry-Immunofluoresence (ICC-IF)
1:2,000 - 1:10,000. Formaldehyde fixation and permeabilization with Triton-X 100 is recommended. Acetone fixation may adversely effect staining.
Flow Cytometry (F)
0.4 µg per 1 X 10^6 cells in a 150 µl volume
Target Sentence
Histone H3 is one of four histone proteins that assemble into an octamer to form the nucleosome core and serve to spool and compact DNA into chromatin. Histones are subject to a complex set of post-translational modifications (acetylation, methylation, phosphorylation, ubiquitination, sumoylation, citrullination, and ADP ribosylation) that are important to the regulation of gene transcription, DNA replication, and DNA repair.
Product Link
http://www.bethyl.com/product/A301-844A
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