A302-757A-T
- Brand
- Bethyl Laboratories
- Application
- IP
- Storage Conditions
- 2 - 8° C
- Host
- Rabbit
- Conjugation
- Unconjugated
- Immunogen
- between 350 and 400
- Buffer
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
- Reactivity
- Human
- Target
- RAP74
- Shelf Life
- 1 year from date of receipt
- Antibody Type
- Polyclonal
- Alternate Names
- BTF4, general transcription factor IIF 74 kDa subunit, general transcription factor IIF subunit 1, general transcription factor IIF, polypeptide 1, 74kDa, RAP74, TF2F1, TFIIF, TFIIF-alpha, transcription initiation factor IIF subunit alpha, transcription initiation factor RAP74
- Format
- Whole IgG
- Presumed Reactivity
- Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat and Bovine
- Product Type
- Primary Antibodies
- Product Sub Types
- Primary Antibodies
- UNSPSC
- 12352203
- Western Blot (WB)
- Not recommended. A302-757A has not performed satisfactorily when used for WB of RAP74 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of RAP74.
- Immunoprecipitation (IP)
- 2 - 5 µg/mg lysate
- Target Sentence
- The general transcription factor, TFIIF, is composed of a heterodimer of a 74 kDa alpha subunit and a 30 kDa beta subunit. The alpha subunit of TFIIF is also called RAP74. In collaboration with TFIIB, TFIIF helps recruit RNA polymerase II to the pre-initiation complex (PIC).
- Product Link
- http://www.bethyl.com/product/A302-757A