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Rabbit anti-RAP74 Antibody, Affinity Purified

Catalog No.
A302-757A-T
Pack Size
  • 100 µl (1000 µg/ml)
  • 10 µl (1000 µg/ml)
Quantity
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Brand:
Specifications
A302-757A-T
Brand
Bethyl Laboratories
Application
IP
Storage Conditions
2 - 8° C
Host
Rabbit
Conjugation
Unconjugated
Immunogen
between 350 and 400
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Reactivity
Human
Target
RAP74
Shelf Life
1 year from date of receipt
Antibody Type
Polyclonal
Alternate Names
BTF4, general transcription factor IIF 74 kDa subunit, general transcription factor IIF subunit 1, general transcription factor IIF, polypeptide 1, 74kDa, RAP74, TF2F1, TFIIF, TFIIF-alpha, transcription initiation factor IIF subunit alpha, transcription initiation factor RAP74
Format
Whole IgG
Presumed Reactivity
Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat and Bovine
Product Type
Primary Antibodies
Product Sub Types
Primary Antibodies
UNSPSC
12352203
Western Blot (WB)
Not recommended. A302-757A has not performed satisfactorily when used for WB of RAP74 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of RAP74.
Immunoprecipitation (IP)
2 - 5 µg/mg lysate
Target Sentence
The general transcription factor, TFIIF, is composed of a heterodimer of a 74 kDa alpha subunit and a 30 kDa beta subunit. The alpha subunit of TFIIF is also called RAP74. In collaboration with TFIIB, TFIIF helps recruit RNA polymerase II to the pre-initiation complex (PIC).
Product Link
http://www.bethyl.com/product/A302-757A
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