Rabbit anti-RBL1 Antibody, Affinity Purified
Brand:
A305-672A-M
- Brand
- Bethyl Laboratories
- Application
- IP
- Storage Conditions
- 2 - 8° C
- Host
- Rabbit
- Conjugation
- Unconjugated
- Immunogen
- Between 1018 and 1068
- Buffer
- Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
- Reactivity
- Human
- Target
- RBL1
- Shelf Life
- 1 year from date of receipt
- Antibody Type
- Polyclonal
- Alternate Names
- 107 kDa retinoblastoma-associated protein, CP107, p107, PRB1, retinoblastoma-like 1, retinoblastoma-like protein 1
- Format
- Whole IgG
- Presumed Reactivity
- Based on 100% sequence identity, this antibody is predicted to react with Mouse
- Product Type
- Primary Antibodies
- Product Sub Types
- Primary Antibodies
- UNSPSC
- 12352203
- Western Blot (WB)
- Not recommended. A305-672A-T has not performed satisfactorily when used for WB of RBL1 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of RBL1.
- Immunoprecipitation (IP)
- 6 - 15 µl/mg lysate
- Target Sentence
- Retinoblastoma-like protein 1 (RBL1) is a key regulator of entry into cell division. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. RBL1 recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. It controls histone H4 'Lys-20' trimethylation. RBL1 probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. It is a potent inhibitor of E2F-mediated trans-activation. RBL1 forms a complex with adenovirus E1A and with SV40 large T antigen and may bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding. RBL1 may act as a tumor suppressor [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P28749].
- Product Link
- http://www.bethyl.com/product/A305-672A-T