Rabbit anti-RNF20 Antibody, Affinity Purified
Brand:
A300-715A-M
- Brand
- Bethyl Laboratories
- Application
- IHC
IP
WB
- Storage Conditions
- 2 - 8° C
- Host
- Rabbit
- Conjugation
- Unconjugated
- Immunogen
- between 125 and 175
- Buffer
- Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
- Reactivity
- Human
- Target
- RNF20
- Shelf Life
- 1 year from date of receipt
- Antibody Type
- Polyclonal
- Alternate Names
- BRE1, BRE1 E3 ubiquitin ligase homolog, BRE1A, BRE1-A, E3 ubiquitin-protein ligase BRE1A, hBRE1, homolog of S. cerevisiae BRE1, RING finger protein 20, RING-type E3 ubiquitin transferase BRE1A
- Format
- Whole IgG
- Presumed Reactivity
- Based on 100% sequence identity, this antibody is predicted to react with Mouse, Chicken and Bovine
- Product Type
- Primary Antibodies
- Product Sub Types
- Primary Antibodies
- UNSPSC
- 12352203
- Western Blot (WB)
- 1:1000
- Immunoprecipitation (IP)
- The antibody contained within A300-715A-T has been qualified for use in immunoprecipitation; however, we recommend using the alternative formulation of this antibody found as product A300-715A.
- Immunohistochemistry (IHC)
- 1:50 - 1:200. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
- Target Sentence
- The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes, and the ligation of ubiquitin to the target protein by E3 enzymes. Ring finger protein 20 (RNF20) is a member of the RING type E3 ubiquitin ligases. RNF20 forms a complex with RNF40 to carry out H2B monoubiquitination in association with hPAF and the UbcH6 ubiquitin E2-conjugating enzyme. H2B monoubiquitination by RNF40/RNF20 has been shown to be associated with increased H3 methylation and gene transcription.
- Product Link
- http://www.bethyl.com/product/A300-715A-T
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