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Rabbit anti-UBF Antibody, Affinity Purified

Catalog No.
A301-859A-T
Pack Size
  • 100 µl (1000 µg/ml)
  • 10 µl (1000 µg/ml)
Quantity
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Brand:
Specifications
A301-859A-T
Brand
Bethyl Laboratories
Application
IP
Storage Conditions
2 - 8° C
Host
Rabbit
Conjugation
Unconjugated
Immunogen
between 546 and 596
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Reactivity
Human
Target
UBF
Shelf Life
1 year from date of receipt
Antibody Type
Polyclonal
Alternate Names
90-kDa nucleolus organizer region autoantigen, autoantigen NOR-90, CONDBA, NOR-90, nucleolar transcription factor 1, UBF, UBF1, UBF-1, UBF2, upstream binding transcription factor, RNA polymerase I, Upstream-binding factor 1
Format
Whole IgG
Presumed Reactivity
Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat and X. laevis
Product Type
Primary Antibodies
Product Sub Types
Primary Antibodies
UNSPSC
12352203
Western Blot (WB)
Not recommended. A301-859A has not performed satisfactorily when used for WB of UBF in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of UBF.
Immunoprecipitation (IP)
2 - 5 µg/mg lysate
Target Sentence
Upstream binding factor (UBF) was originally identified as a multi-HMG (high mobility group)-box DNA binding domain protein that could enhance the in vitro assembly of the RNA polymerase I initiation complex. UBF is a transcription factor that recognizes the ribosomal RNA gene promoter required for the expression of 18S, 5.8S, and 28S ribosomal RNAs.
Product Link
http://www.bethyl.com/product/A301-859A
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