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HEPES

Catalog No.
BIO-1825-100g
N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid HEPES is a buffering agent used in biochemistry and molecular biology. It is a zwitterionic at biological pH and is most effective at pH range of 6.8 – 8.2. It is commonly used in cell culture media. It is also suitable for use in the bicinchoninic acid (BCA) assay.
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Pack Size
  • 100g
  • 500g
  • 1kg
  • 5kg
Quantity
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Specifications
BIO-1825-100g
Brand
1st BASE
CAS
7365-45-9
Grade
Ultra Pure Grade
Storage Conditions
Store at room temperature. Protect from moisture.
Molecular Formula
C₈H₁₈N₂O₄S
Molecular Weight
238.3
Appearance
White Powder
Assay
min. 99.5 %
Residue on ignition (as sulfate)
≤ 0.1 %
Absorbance
≤ 0.05 @260 nm (1mol/L, 1 cm); ≤ 0.05 @280 nm (1mol/L, 1 cm)
Loss on Drying
≤ 1.0 %
pKa (20°C)
7.35 – 7.69
Note
Avoid contact and avoid breathing dust. Minimize dust.
Do not inhale or ingest. For lab use only.
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