10×A-attachment Mix

10 x A-attachment mix is a reagent comprising anti-KOD DNA polymerase antibody specific to KOD 3'→5' exonuclease activity (proof-reading activity)(1), as well as Taq DNA polymerase, which exhibits terminal transferase activity. PCR products from KOD-Plus- [Code No. KOD-201] and KOD FX [Code No. KFX-101] possess blunt ends due to 3'→5' exonuclease activity of the KOD DNA polymerase. The 10 x A-attachment mix allows for PCR products to acquire overhanging dA at the 3'-ends. Products with 3'-dA overhangs can be directly cloned into arbitrary T-vectors using ligation reagents, such as Ligation high Ver.2 [Code No. LGK-201].