MyTaq One-Step RT-PCR Kit

MyTaq™ One-Step RT-PCR Kit has been designed for extremely sensitive and highly reproducible first-stand cDNA synthesis and subsequent PCR in a single tube (fig. 1). The kit contains the latest advances in buffer chemistry, including Meridian´s ultra-pure dNTPs, together with reverse transcriptase (RT) and our new generation of very high performance, antibody-mediated hotstart DNA polymerase (MyTaq HS). This ensures that MyTaq OneStep RT-PCR Kit produces fast, highly-specific and ultrasensitive products for downstream applications. MyTaq One-Step Kit consists of reverse transcriptase, 2x MyTaq HS Mix and a potent RNase Inhibitor, RiboSafe, that are added together to create a simple to use all-in-one mix. The kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative, semiquantitative or quantitative analysis of RNA transcription levels, and the one-step format is also perfect for the synthesis of doublestranded cDNA products for subsequent gene-expression analysis. The cDNA can be synthesized with starting amounts of RNA template from 3 pg to 1 μg, over a broad temperature range (up to 50°C to overcome secondary structure and GCrich sequences), prior to heating to 95°C to inactivate reverse transcriptase and simultaneously to activate the MyTaq™ HS.