Rabbit anti-BAD Antibody, Affinity Purified
Brand:
A302-384A-T
- Brand
- Bethyl Laboratories
- Application
- IHC
IP
- Storage Conditions
- 2 - 8° C
- Host
- Rabbit
- Conjugation
- Unconjugated
- Immunogen
- between 1 and 50
- Buffer
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
- Reactivity
- Human, Mouse
- Target
- BAD
- Shelf Life
- 1 year from date of receipt
- Antibody Type
- Polyclonal
- Alternate Names
- BAD, BBC2, bcl2 antagonist of cell death, BCL2-antagonist of cell death protein, bcl2-associated agonist of cell death, bcl-2-binding component 6, BCL2-binding component 6, BCL2-binding protein, BCL2L8, bcl2-L-8, bcl-2-like protein 8, BCL-X/BCL-2 binding protein, bcl-XL/Bcl-2-associated death promoter
- Format
- Whole IgG
- Presumed Reactivity
- Based on 100% sequence identity, this antibody is predicted to react with Rat
- Product Type
- Primary Antibodies
- Product Sub Types
- Primary Antibodies
- UNSPSC
- 12352203
- Western Blot (WB)
- Not recommended. A302-384A has not performed satisfactorily when used for WB of BAD in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of BAD.
- Immunoprecipitation (IP)
- 5 - 10 µg/mg lysate
- Immunohistochemistry (IHC)
- 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
- Target Sentence
- BAD (Bcl2 agonist of cell death) is a member of the Bcl-2 family. Bcl-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with Bcl-xL and Bcl-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein [taken from NCBI Entrez Gene (GeneID:572)].
- Product Link
- http://www.bethyl.com/product/A302-384A
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