![Rabbit anti-BRD4 Recombinant Monoclonal Antibody [BL-149-2H5]](https://www.axilscientific.com/10682-superlarge_default/rabbit-anti-brd4-recombinant-monoclonal-antibody-bl-149-2h5-.jpg)
Rabbit anti-BRD4 Recombinant Monoclonal Antibody [BL-149-2H5]
Brand:
A700-004-T
- Brand
- Bethyl Laboratories
- Application
- ChIP-Seq
F
ICC
ICC-IF
IHC
IP
WB
- Storage Conditions
- 2 - 8° C
- Host
- Rabbit
- Conjugation
- Unconjugated
- Immunogen
- Between 1312 and 1362
- Buffer
- Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
- Reactivity
- Human, Mouse
- Target
- BRD4
- Shelf Life
- 1 year from date of receipt
- Antibody Type
- Recombinant Monoclonal
- Alternate Names
- bromodomain-containing protein 4, CAP, chromosome-associated protein, HUNK1, HUNKI, MCAP, Protein HUNK1
- Format
- Whole IgG
- Product Type
- Primary Antibodies
- Product Sub Types
- Primary Antibodies
- UNSPSC
- 12352203
- Western Blot (WB)
- 1:1000
- Immunoprecipitation (IP)
- 20 µl/mg lysate
- Immunohistochemistry (IHC)
- 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
- Immunocytochemistry (ICC)
- 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
- Immunocytochemistry-Immunofluoresence (ICC-IF)
- 1:25 - 1:250. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
- ChIP-Seq
- A previous lot has qualified for ChIP-Seq. 10 - 40 µl per 30 µg chromatin.
- Flow Cytometry (F)
- Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells.
- Target Sentence
- Bromodomain-containing protein 4 (BRD4) contains 2 bromo-domains. Bromo-domains are found in chromatin-associating proteins and can interact specifically with acetylated lysine. BRD4 is described as a chromatin adaptor and has been shown to bind acetylated chromatin. It was originally identified as MCAP (mitotic chromosome associated protein) due to its association with mitotic chromosomes. BRD4 also associates with several transcription complexes such as the Mediator complexes and P-TEFb complexes and is considered to function as a transcriptional co-factor.
- Product Link
- http://www.bethyl.com/product/A700-004