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Rabbit anti-HIF2-alpha Recombinant Monoclonal Antibody [BL-95-1A2]
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Brand:
A700-003-T
- Brand
- Bethyl Laboratories
- Application
- ChIP-Seq
ICC
ICC-IF
IHC
IP
WB
- Storage Conditions
- 2 - 8° C
- Host
- Rabbit
- Conjugation
- Unconjugated
- Immunogen
- between 400 and 450
- Buffer
- Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
- Reactivity
- Human
- Target
- HIF2-alpha
- Shelf Life
- 1 year from date of receipt
- Antibody Type
- Recombinant Monoclonal
- Alternate Names
- basic-helix-loop-helix-PAS protein MOP2, bHLHe73, class E basic helix-loop-helix protein 73, ECYT4, endothelial PAS domain-containing protein 1, EPAS-1, HIF-1-alpha-like factor, HIF-1alpha-like factor, HIF2A, HIF-2-alpha, HIF2-alpha, HLF, hypoxia-inducible factor 2 alpha, Hypoxia-inducible factor 2-alpha, member of PAS protein 2, MOP2, PAS domain-containing protein 2, PASD2
- Format
- Whole IgG
- Product Type
- Primary Antibodies
- Product Sub Types
- Primary Antibodies
- UNSPSC
- 12352203
- Western Blot (WB)
- 1:1000
- Immunoprecipitation (IP)
- 5 - 20 µl/mg lysate
- Immunohistochemistry (IHC)
- 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes using a pressure cooker is recommended for FFPE tissue sections. Overnight incubations are suggested.
- Immunocytochemistry (ICC)
- 1:200 - 1:1,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
- Immunocytochemistry-Immunofluoresence (ICC-IF)
- 1:100 - 1:500. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
- ChIP-Seq
- A previous lot has qualified for ChIP-Seq. 10 - 40 µl per 30 µg chromatin.
- Target Sentence
- The gene EPAS1 encodes for the transcription factor endothelial PAS domain-containing protein 1, and is induced as oxygen levels fall. Commonly called hypoxia inducible factor 2A (HIF2a), this protein is involved in the induction of oxygen regulated genes, binding to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. An important cancer biomarker, HIF2a may also play a role in Tibetan high-altitude adaptation (Am J Hum Genet. 2015 Jul 2; 97(1): 54–66.) and mutations in the EPAS1 gene are associated with erythrocytosis familial type 4 [taken from NCBI Entrez Gene (Gene ID: 2034) and UniProtKB (Entry: Q99814)].
- Product Link
- http://www.bethyl.com/product/A700-003