A304-376A-M
- Brand
- Bethyl Laboratories
- Application
- ICC-IF
IHC
IP
WB
- Storage Conditions
- 2 - 8° C
- Host
- Rabbit
- Conjugation
- Unconjugated
- Immunogen
- Between 1254 and 1304
- Buffer
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
- Reactivity
- Human
- Target
- CD45
- Shelf Life
- 1 year from date of receipt
- Antibody Type
- Polyclonal
- Alternate Names
- B220, CD antigen CD45, CD45, CD45 antigen, CD45R, GP180, LCA, L-CA, Leukocyte common antigen, LY5, protein tyrosine phosphatase, receptor type, c polypeptide, receptor-type tyrosine-protein phosphatase C, T200, T200 glycoprotein, T200 leukocyte common antigen
- Format
- Whole IgG
- Product Type
- Primary Antibodies
- Product Sub Types
- Primary Antibodies
- UNSPSC
- 12352203
- Western Blot (WB)
- 1:2,000 - 1:10,000
- Immunoprecipitation (IP)
- 2 - 10 µg/mg lysate
- Immunohistochemistry (IHC)
- 1:1,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
- Immunocytochemistry-Immunofluoresence (ICC-IF)
- 1:1,000 - 1:5,000. Acetone or Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
- Target Sentence
- CD45 is a protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. CD45 acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 and FYN [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P08575].
- Product Link
- http://www.bethyl.com/product/A304-376A