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Rabbit anti-HIF1-alpha Recombinant Monoclonal Antibody [BL-124-3F7]
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Brand:
A700-001-T
- Brand
- Bethyl Laboratories
- Application
- ChIP-Seq
ICC
IHC
IP
WB
- Storage Conditions
- 2 - 8° C
- Host
- Rabbit
- Conjugation
- Unconjugated
- Immunogen
- between 433 and 826
- Buffer
- Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
- Reactivity
- Human
- Target
- HIF1-alpha
- Shelf Life
- 1 year from date of receipt
- Antibody Type
- Recombinant Monoclonal
- Alternate Names
- ARNT-interacting protein, basic-helix-loop-helix-PAS protein MOP1, bHLHe78, class E basic helix-loop-helix protein 78, HIF1, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1-ALPHA, hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), hypoxia-inducible factor 1 alpha isoform I.3, hypoxia-inducible factor 1-alpha, hypoxia-inducible factor1alpha, member of PAS protein 1, member of PAS superfamily 1, MOP1, PAS domain-containing protein 8, PASD8
- Format
- Whole IgG
- Product Type
- Primary Antibodies
- Product Sub Types
- Primary Antibodies
- UNSPSC
- 12352203
- Western Blot (WB)
- 1:1000
- Immunoprecipitation (IP)
- 20 µl/ mg lysate
- Immunohistochemistry (IHC)
- 1:100 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes using a pressure cooker is recommended for FFPE tissue sections.
- Immunocytochemistry (ICC)
- 1:100 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes using a pressure cooker is recommended for FFPE cell sections.
- ChIP-Seq
- 10 µl per 30 µg of chromatin
- Target Sentence
- Hypoxia-inducible factor-1 (HIF1) is a basic helix-loop-helix (bHLH)-PER-ARNT-sim (PAS) transcription factor that plays a role in the cellular response to hypoxia. HIF1 is a heterodimer of an HIF1-alpha and HIF1-beta subunit. During normoxia, HIF-1alpha is regulated by ubiquitination and interaction with the von-Hippel-Lindau tumor suppressor protein (pVHL).
- Product Link
- http://www.bethyl.com/product/A700-001