Rabbit anti-PCNA Antibody, Affinity Purified
Brand:
A300-276A-M
- Brand
- Bethyl Laboratories
- Application
- F
IHC
IP
WB
- Storage Conditions
- 2 - 8° C
- Host
- Rabbit
- Conjugation
- Unconjugated
- Immunogen
- Between 75 and 125
- Buffer
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
- Reactivity
- Human, Mouse
- Target
- PCNA
- Shelf Life
- 1 year from date of receipt
- Antibody Type
- Polyclonal
- Alternate Names
- ATLD2, cyclin, DNA polymerase delta auxiliary protein, PCNA, proliferating cell nuclear antigen
- Format
- Whole IgG
- Presumed Reactivity
- Based on 100% sequence identity, this antibody is predicted to react with Rat, Chicken and Bovine
- Product Type
- Primary Antibodies
- Product Sub Types
- Primary Antibodies
- UNSPSC
- 12352203
- Western Blot (WB)
- 1:1,000 - 1:10,000
- Immunoprecipitation (IP)
- 2 - 5 µg/mg lysate
- Immunohistochemistry (IHC)
- 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
- Flow Cytometry (F)
- 0.03 µg per 1 X 10^6 cells in a 150 µl volume
- Target Sentence
- PCNA (Proliferating Cell Nuclear Antigen) was first identified as a factor expressed in the nuclei of replicating cells. PCNA plays a role in both DNA synthesis and repair. PCNA functions as a processivity factor for DNA polymerase by acting as a DNA clamp that tethers the polymerase catalytic units to the DNA template. The structure of PCNA is that of a trimeric ring that encircles and slides along replicating DNA. PCNA interacts with and is regulated by several proteins involved in cell cycle control and check point processes.
- Product Link
- http://www.bethyl.com/product/A300-276A
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